TY - JOUR
T1 - Whey protein isolate modulates beta-carotene bioaccessibility depending on gastro-intestinal digestion conditions
AU - Iddir, Mohammed
AU - Degerli, Celal
AU - Dingeo, Giulia
AU - Desmarchelier, Charles
AU - Schleeh, Thomas
AU - Borel, Patrick
AU - Larondelle, Yvan
AU - Bohn, Torsten
N1 - Funding Information:
The authors are grateful for the support of the Fonds National de la Recherche Luxembourg (Grant No. C16/BM/11320230 ). The insights obtained from the EU-COST actions EUROCAROTEN (CA15136) and POSITIVE (FA 1403) are much appreciated. The authors thank Yvan Devaux, head of cardiovascular research unit, for placing facilities at our disposal. The help of Bernadette Leners during digestions, fatty acid titrations and SDS-page is much appreciated.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Carotenoids are lipophilic phytochemicals; their intake has been associated with reduced chronic diseases. However, their absorption depends on emulsification during digestion and incorporation into mixed micelles, requiring digestive enzymes, gastric peristalsis, bile, and dietary lipids. In this study, we investigated whether whey-protein-isolate (WPI), a commonly consumed protein source, can modulate β-carotene bioaccessibility in vitro, especially under incomplete digestive conditions, i.e. under low digestive enzyme concentrations. Thus, amounts of pepsin, pancreatin, bile, co-digested lipids and kinetic energy and gastric digestion time were modified, and WPI at concentrations equivalent to 0/25/50% of the protein recommended dietary allowance (approx. 60 g/d) were added to β-carotene dissolved in oil. WPI enhanced bioaccessibility by up to 20% (p < 0.001), especially under higher simulated peristalsis or reduced amount of dietary lipids. Conversely, they impaired bioaccessibility to one third (p < 0.001) under incomplete digestive conditions. WPI modulated β-carotene bioaccessibility depending on digestive conditions.
AB - Carotenoids are lipophilic phytochemicals; their intake has been associated with reduced chronic diseases. However, their absorption depends on emulsification during digestion and incorporation into mixed micelles, requiring digestive enzymes, gastric peristalsis, bile, and dietary lipids. In this study, we investigated whether whey-protein-isolate (WPI), a commonly consumed protein source, can modulate β-carotene bioaccessibility in vitro, especially under incomplete digestive conditions, i.e. under low digestive enzyme concentrations. Thus, amounts of pepsin, pancreatin, bile, co-digested lipids and kinetic energy and gastric digestion time were modified, and WPI at concentrations equivalent to 0/25/50% of the protein recommended dietary allowance (approx. 60 g/d) were added to β-carotene dissolved in oil. WPI enhanced bioaccessibility by up to 20% (p < 0.001), especially under higher simulated peristalsis or reduced amount of dietary lipids. Conversely, they impaired bioaccessibility to one third (p < 0.001) under incomplete digestive conditions. WPI modulated β-carotene bioaccessibility depending on digestive conditions.
KW - Carotenoids
KW - Digestion
KW - Emulsion
KW - Lipid droplets
KW - Micellization
KW - Peristalsis
KW - Proteins
UR - http://www.scopus.com/inward/record.url?scp=85063998410&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2019.04.003
DO - 10.1016/j.foodchem.2019.04.003
M3 - Article
C2 - 31006454
AN - SCOPUS:85063998410
SN - 0308-8146
VL - 291
SP - 157
EP - 166
JO - Food Chemistry
JF - Food Chemistry
ER -