Validation of a quick PCR method suitable for direct sequencing: Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety

Marine Pallez; Matias Pasquali; Torsten Bohn; Lucien Hoffmann; Marco Beyer

Validation of a quick PCR method suitable for direct sequencing: Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety

Marine Pallez, Matias Pasquali^*, Torsten Bohn, Lucien Hoffmann, Marco Beyer

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.

Original language	English
Pages (from-to)	351-358
Number of pages	8
Journal	Food Technology and Biotechnology
Volume	52
Issue number	3
Publication status	Published - 2014
Externally published	Yes

Keywords

EF1-α
Fusarium culmorum
Fusarium graminearum
Microwave DNA extraction

Cite this

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title = "Validation of a quick PCR method suitable for direct sequencing: Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety",

abstract = "Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.",

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author = "Marine Pallez and Matias Pasquali and Torsten Bohn and Lucien Hoffmann and Marco Beyer",

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language = "English",

volume = "52",

pages = "351--358",

journal = "Food Technology and Biotechnology",

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Validation of a quick PCR method suitable for direct sequencing: Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety. / Pallez, Marine; Pasquali, Matias; Bohn, Torsten et al.
In: Food Technology and Biotechnology, Vol. 52, No. 3, 2014, p. 351-358.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Validation of a quick PCR method suitable for direct sequencing

T2 - Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety

AU - Pallez, Marine

AU - Pasquali, Matias

AU - Bohn, Torsten

AU - Hoffmann, Lucien

AU - Beyer, Marco

PY - 2014

Y1 - 2014

N2 - Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.

AB - Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.

KW - EF1-α

KW - Fusarium culmorum

KW - Fusarium graminearum

KW - Microwave DNA extraction

UR - http://www.scopus.com/inward/record.url?scp=84901410915&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84901410915

SN - 1330-9862

VL - 52

SP - 351

EP - 358

JO - Food Technology and Biotechnology

JF - Food Technology and Biotechnology

IS - 3

ER -

Validation of a quick PCR method suitable for direct sequencing: Identification of Fusarium fungal species and chemotypes for preventive approaches in food safety

Abstract

Keywords

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