Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.
|Number of pages||8|
|Journal||Food Technology and Biotechnology|
|Publication status||Published - 2014|
- Fusarium culmorum
- Fusarium graminearum
- Microwave DNA extraction