Using PrISMa to reveal the interactome of the human claudins family

Daniel Perez-Hernandez; Lorena Suarez-Artiles; Mattson S.O. Jones; Gunnar Dittmar

doi:10.1016/j.xpro.2023.102549

Using PrISMa to reveal the interactome of the human claudins family

Daniel Perez-Hernandez, Lorena Suarez-Artiles, Mattson S.O. Jones, Gunnar Dittmar^*

^*Corresponding author for this work

Proteomics of Cellular Signaling

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.¹

Original language	English
Article number	102549
Journal	STAR Protocols
Volume	4
Issue number	4
Early online date	26 Sept 2023
DOIs	https://doi.org/10.1016/j.xpro.2023.102549
Publication status	Published - 15 Dec 2023

Keywords

High-throughput Screening
Mass Spectrometry
Protein Biochemistry
Proteomics

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10.1016/j.xpro.2023.102549Licence: CC BY

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title = "Using PrISMa to reveal the interactome of the human claudins family",

abstract = "Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1",

keywords = "High-throughput Screening, Mass Spectrometry, Protein Biochemistry, Proteomics",

author = "Daniel Perez-Hernandez and Lorena Suarez-Artiles and Jones, {Mattson S.O.} and Gunnar Dittmar",

note = "Funding Information: M.J. was supported by the FNR CORE grant HIFReg ( C21/BM/15839877 ) to G.D.. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

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AU - Perez-Hernandez, Daniel

AU - Suarez-Artiles, Lorena

AU - Jones, Mattson S.O.

AU - Dittmar, Gunnar

PY - 2023/12/15

Y1 - 2023/12/15

N2 - Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1

AB - Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1

KW - High-throughput Screening

KW - Mass Spectrometry

KW - Protein Biochemistry

KW - Proteomics

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JO - STAR Protocols

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Using PrISMa to reveal the interactome of the human claudins family

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Keywords

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