Using PrISMa to reveal the interactome of the human claudins family

Daniel Perez-Hernandez, Lorena Suarez-Artiles, Mattson S.O. Jones, Gunnar Dittmar*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review


Here, we provide a protocol for the systematic screening of protein-protein interactions mediated by short linear motifs using the Protein Interaction Screen on a peptide Matrix (PrISMa) technique. We describe how to pull down interacting proteins in a parallelized manner and identify them by mass spectrometry. Finally, we describe a bioinformatic workflow necessary to identify highly probable interaction partners in the large-scale dataset. We describe the application of this method for the transient interactome of the claudin protein family. For complete details on the use and execution of this protocol, please refer to Suarez-Artiles et al.1

Original languageEnglish
Article number102549
JournalSTAR Protocols
Issue number4
Early online date26 Sept 2023
Publication statusPublished - 15 Dec 2023


  • High-throughput Screening
  • Mass Spectrometry
  • Protein Biochemistry
  • Proteomics


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