TY - JOUR
T1 - Use of Autostitch for automatic stitching of microscope images
AU - Ma, Bin
AU - Zimmermann, Timo
AU - Rohde, Manfred
AU - Winkelbach, Simon
AU - He, Feng
AU - Lindenmaier, Werner
AU - Dittmar, Kurt E.J.
N1 - Funding Information:
We acknowledge the financial supports from University Hospital of Ulm, Germany and continual support of the Advanced Light Microscopy Facility of EMBL by Visitron GmbH. We also want to thank Wayne Rasband for the Image J and Matthew Brown for the Autostitch program.
PY - 2007/7
Y1 - 2007/7
N2 - Image stitching is the process of combining multiple images to produce a panorama or larger image. In many biomedical studies, including those of cancer and infection, the use of this approach is highly desirable in order to acquire large areas of certain structures or whole sections, while retaining microscopic resolution. In this study, we describe the application of Autostitch, viz. software that is normally used for the generation of panoramas in photography, in the seamless stitching of microscope images. First, we tested this software on image sets manually acquired by normal light microscopy and compared the performance with a manual stitching approach performed with Paint Shop Pro. Secondly, this software was applied to an image stack acquired by an automatic microscope. The stitching results were then compared with that generated by a self-programmed rectangular tiling macro integrated in Image J. Thirdly, this program was applied in the image stitching of images from electron microscopy. Thus, the automatic stitching program described here may find applications in convenient image stitching and virtual microscopy in the biomedical research.
AB - Image stitching is the process of combining multiple images to produce a panorama or larger image. In many biomedical studies, including those of cancer and infection, the use of this approach is highly desirable in order to acquire large areas of certain structures or whole sections, while retaining microscopic resolution. In this study, we describe the application of Autostitch, viz. software that is normally used for the generation of panoramas in photography, in the seamless stitching of microscope images. First, we tested this software on image sets manually acquired by normal light microscopy and compared the performance with a manual stitching approach performed with Paint Shop Pro. Secondly, this software was applied to an image stack acquired by an automatic microscope. The stitching results were then compared with that generated by a self-programmed rectangular tiling macro integrated in Image J. Thirdly, this program was applied in the image stitching of images from electron microscopy. Thus, the automatic stitching program described here may find applications in convenient image stitching and virtual microscopy in the biomedical research.
KW - Automatic microscopy
KW - Autostitch
KW - Image masaicing
KW - Image stitching
KW - Virtual microscopy
UR - http://www.scopus.com/inward/record.url?scp=33947210785&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/17045805
U2 - 10.1016/j.micron.2006.07.027
DO - 10.1016/j.micron.2006.07.027
M3 - Article
C2 - 17045805
AN - SCOPUS:33947210785
SN - 0968-4328
VL - 38
SP - 492
EP - 499
JO - Micron
JF - Micron
IS - 5
ER -