TY - JOUR
T1 - Ultra performance liquid chromatography – tandem mass spectrometer method applied to the analysis of both thyroid and steroid hormones in human hair
AU - Grova, N.
AU - Wang, X.
AU - Hardy, E. M.
AU - Palazzi, P.
AU - Chata, C.
AU - Appenzeller, B. M.R.
N1 - Funding Information:
This work was supported by the Luxembourg ? Minist?re de l'Enseignement Sup?rieur et de la Recherche ?.
Publisher Copyright:
© 2019
PY - 2020/2/8
Y1 - 2020/2/8
N2 - Hair is increasingly used as a biological matrix of interest for the assessment of hormone secretion over extended periods of time. This study described the development and the validation of a sensitive UPLC-MS/MS method for simultaneous analysis of steroid and thyroid hormones in human hair. The gradient designed in this method enables to obtain a satisfactory separation of 9 hormones of interest: cortisol, cortisone, THE, THF, α-THF, triiodothyronine (T3) and thyroxine (T4), estradiol, and testosterone. Several methodological parameters of extraction (such as the used of “cut hair” versus “pulverized hair”, the extraction time, the incubation solvent purification on SPE column and hydrolysis) that may influence the determination of hormones levels in human hair, have thus been tested here. Therefore, the results obtained highlighted the necessity of using a C18 SPE purification method for the determination of both steroid and thyroid hormones in hair. This method allows reaching suitable levels of sensitivity for cortisol and cortisone since the results obtained pointed out concentration levels of cortisol in hair of volunteers similar to those observed in the literature. This method could also offer an important impact in the field of hormone analysis since it allows, for the first time, the quantification of both T3 and T4 in human hair.
AB - Hair is increasingly used as a biological matrix of interest for the assessment of hormone secretion over extended periods of time. This study described the development and the validation of a sensitive UPLC-MS/MS method for simultaneous analysis of steroid and thyroid hormones in human hair. The gradient designed in this method enables to obtain a satisfactory separation of 9 hormones of interest: cortisol, cortisone, THE, THF, α-THF, triiodothyronine (T3) and thyroxine (T4), estradiol, and testosterone. Several methodological parameters of extraction (such as the used of “cut hair” versus “pulverized hair”, the extraction time, the incubation solvent purification on SPE column and hydrolysis) that may influence the determination of hormones levels in human hair, have thus been tested here. Therefore, the results obtained highlighted the necessity of using a C18 SPE purification method for the determination of both steroid and thyroid hormones in hair. This method allows reaching suitable levels of sensitivity for cortisol and cortisone since the results obtained pointed out concentration levels of cortisol in hair of volunteers similar to those observed in the literature. This method could also offer an important impact in the field of hormone analysis since it allows, for the first time, the quantification of both T3 and T4 in human hair.
KW - Hair analysis
KW - Hormones
KW - Method
KW - Steroids
KW - Thyroids
KW - UPLC-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=85074480363&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2019.460648
DO - 10.1016/j.chroma.2019.460648
M3 - Article
C2 - 31679711
AN - SCOPUS:85074480363
SN - 0021-9673
VL - 1612
JO - Journal of Chromatography A
JF - Journal of Chromatography A
M1 - 460648
ER -