@article{8f3e8d6613f7436089aa45ef9883e45e,
title = "Transcriptional variations in the wider peritumoral tissue environment of pancreatic cancer",
abstract = "Transcriptional profiling was performed on 452 RNA preparations isolated from various types of pancreatic tissue from tumour patients and healthy donors, with a particular focus on peritumoral samples. Pancreatic ductal adenocarcinomas (PDAC) and cystic tumours were most different in these non-tumorous tissues surrounding them, whereas the actual tumours exhibited rather similar transcript patterns. The environment of cystic tumours was transcriptionally nearly identical to normal pancreas tissue. In contrast, the tissue around PDAC behaved a lot like the tumour, indicating some kind of field defect, while showing far less molecular resemblance to both chronic pancreatitis and healthy tissue. This suggests that the major pathogenic difference between cystic and ductal tumours may be due to their cellular environment rather than the few variations between the tumours. Lack of correlation between DNA methylation and transcript levels makes it unlikely that the observed field defect in the peritumoral tissue of PDAC is controlled to a large extent by such epigenetic regulation. Functionally, a strikingly large number of autophagy-related transcripts was changed in both PDAC and its peritumoral tissue, but not in other pancreatic tumours. A transcription signature of 15 autophagy-related genes was established that permits a prognosis of survival with high accuracy and indicates the role of autophagy in tumour biology.",
keywords = "disease prognosis, pancreatic cancer, peritumoral tissue, survival, transcript variations",
author = "Bauer, {Andrea S.} and Nazarov, {Petr V.} and Giese, {Nathalia A.} and Stefania Beghelli and Anette Heller and William Greenhalf and Eithne Costello and Arnaud Muller and Melanie Bier and Oliver Strobel and Thilo Hackert and Laurent Vallar and Aldo Scarpa and B{\"u}chler, {Markus W.} and Neoptolemos, {John P.} and Stephanie Kreis and Hoheisel, {J{\"o}rg D.}",
note = "Funding Information: Key words: pancreatic cancer, peritumoral tissue, transcript variations, survival, disease prognosis A.S.B., P.V.N. and N.A.G. contributed equally and should be considered first authors. Conflict of Interest: The authors declare that they have no conflict of interest. Grant sponsor: German Federal Ministry of Education and Research (BMBF); Grant numbers: 01GS08117 (J.D.H.), 01GS08114 (N.A.G. and M.W.B.) and 01ZX1305C (N.A.G., T.H. and O.S.); Grant sponsor: Heidelberger Stiftung Chirurgie (EPZ-Pancobank) DOI: 10.1002/ijc.31087 This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. History: Received 17 Oct 2016; Accepted 30 Aug 2017; Online 6 Oct 2017 Correspondence to: Jorg D. Hoheisel, Functional Genome Analysis, German Cancer Research Centre (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany, Tel.: 149-6221-424680, Fax: 149-6221-424687, E-mail:
[email protected] Publisher Copyright: {\textcopyright} 2017 The Authors International Journal of Cancer published by John Wiley & Sons Ltd on behalf of UICC.",
year = "2018",
month = mar,
day = "1",
doi = "10.1002/ijc.31087",
language = "English",
volume = "142",
pages = "1010--1021",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "John Wiley and Sons Inc",
number = "5",
}