Transcriptional and post-transcriptional regulation of glutathione S-transferase P1 expression during butyric acid-induced differentiation of K562 cells

Michael Schnekenburger, Franck Morceau, Estelle Henry, Romain Blasius, Mario Dicato, Chantal Trentesaux, Marc Diederich*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Over-expression of glutathione S-transferase P1 is related to chemotherapeutic drug resistance as well as to differentiation of human erythroleukemia cells. In opposition to previously described differentiating inducers which enhance the GST-resistance phenotype, time- and concentration-dependent activation of both erythroid and megakaryocytic differentiation pathways by butyric acid progressively diminished GSTP1 mRNA expression. GSTP1 mRNA expression decreased by 25% (p < 0.01) and 64% (p < 0.01) in 1 mM and 2 mM butyric acid-differentiated K562 cells, respectively. These results were associated to both a reduction of GATA-1 binding activity to the GSTP1 promoter and to a posttranscriptional destabilization of GSTP1 mRNA in a concentration dependent manner. Indeed, GSTP1 mRNA half-life decreased from 43.8 to 36.2 h and 12.6 h in 1 mM- and 2 mM-treated cells, respectively.

Original languageEnglish
Pages (from-to)561-568
Number of pages8
JournalLeukemia Research
Volume30
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

Keywords

  • Butyric acid
  • Erythroid and megakaryocytic differentiation
  • GATA-1
  • GSTP1
  • K562

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