Abstract
Over-expression of glutathione S-transferase P1 is related to chemotherapeutic drug resistance as well as to differentiation of human erythroleukemia cells. In opposition to previously described differentiating inducers which enhance the GST-resistance phenotype, time- and concentration-dependent activation of both erythroid and megakaryocytic differentiation pathways by butyric acid progressively diminished GSTP1 mRNA expression. GSTP1 mRNA expression decreased by 25% (p < 0.01) and 64% (p < 0.01) in 1 mM and 2 mM butyric acid-differentiated K562 cells, respectively. These results were associated to both a reduction of GATA-1 binding activity to the GSTP1 promoter and to a posttranscriptional destabilization of GSTP1 mRNA in a concentration dependent manner. Indeed, GSTP1 mRNA half-life decreased from 43.8 to 36.2 h and 12.6 h in 1 mM- and 2 mM-treated cells, respectively.
Original language | English |
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Pages (from-to) | 561-568 |
Number of pages | 8 |
Journal | Leukemia Research |
Volume | 30 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2006 |
Externally published | Yes |
Keywords
- Butyric acid
- Erythroid and megakaryocytic differentiation
- GATA-1
- GSTP1
- K562