Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: Bioaccessibility and potential uptake

Jaouad Bouayed*, Lucien Hoffmann, Torsten Bohn

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

459 Citations (Scopus)

Abstract

In the present study, an in vitro model simulating gastrointestinal (GI) digestion, including dialysability, was adapted to assess free soluble polyphenols from apples (four varieties). Results indicated that polyphenol release was mainly achieved during the gastric phase (ca. 65% of phenolics and flavonoids), with a slight further release (<10%) during intestinal digestion. Anthocyanins present after the gastric phase (1.04-1.14 mg/100 g) were not detectable following intestinal digestion. Dialysis experiments employing a semipermeable cellulose membrane, presenting a simplified model of the epithelial barrier, showed that free soluble dialysable polyphenols and flavonoids were 55% and 44% of native concentrations, respectively, being approximately 20% and 30% lower than that of the GI digesta. Similar results were found for the antioxidant capacity of dialysable antioxidants, being 57% and 46% lower compared to total antioxidants in fresh apples (FRAP and ABTS test, respectively). It is suggested that some polyphenols are bound to macromolecular compounds that are non-dialysable, that the presented method allowed the study of free soluble polyphenols available for further uptake, and that both chemical extraction and concentrations in final digesta would overestimate polyphenol availability.

Original languageEnglish
Pages (from-to)14-21
Number of pages8
JournalFood Chemistry
Volume128
Issue number1
DOIs
Publication statusPublished - 1 Sep 2011
Externally publishedYes

Keywords

  • Anthocyanins
  • Antioxidant activity
  • Antioxidants
  • Apples
  • Bioaccessibility
  • Dialysability
  • Flavonoids
  • Gastro-intestinal digestion
  • Polyphenols

Fingerprint

Dive into the research topics of 'Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: Bioaccessibility and potential uptake'. Together they form a unique fingerprint.

Cite this