TY - JOUR
T1 - Three-dimensional growth of glial cell lines affects growth factor and growth factor receptor mRNA levels
AU - Ness, Gro Oddveig
AU - Pedersen, Paal Henning
AU - Bjerkvig, Rolf
AU - Laerum, Ole Didrik
AU - Lillehaug, Johan R.
PY - 1994/9
Y1 - 1994/9
N2 - We have studied, at the mRNA level, the influence of various defined growth conditions on the expression of TGF-α, PDGF-BB, EGF-R, PDGF-Rα, and PDGF-Rβ in five different glioma cell lines (D-37MG, 54MG, D-263MG, GaMG, and U-251MG). RNA isolated from logarithmically growing, confluent monolayer cells or multicellular spheroids was analyzed. Northern blot experiments show that with a few exceptions, specific mRNA steady-state levels were considerably higher in cells grown in a three-dimensional organization relative to cells in the logarithmic growth phase.
AB - We have studied, at the mRNA level, the influence of various defined growth conditions on the expression of TGF-α, PDGF-BB, EGF-R, PDGF-Rα, and PDGF-Rβ in five different glioma cell lines (D-37MG, 54MG, D-263MG, GaMG, and U-251MG). RNA isolated from logarithmically growing, confluent monolayer cells or multicellular spheroids was analyzed. Northern blot experiments show that with a few exceptions, specific mRNA steady-state levels were considerably higher in cells grown in a three-dimensional organization relative to cells in the logarithmic growth phase.
UR - http://www.scopus.com/inward/record.url?scp=0028015913&partnerID=8YFLogxK
U2 - 10.1006/excr.1994.1277
DO - 10.1006/excr.1994.1277
M3 - Article
AN - SCOPUS:0028015913
SN - 0014-4827
VL - 214
SP - 433
EP - 436
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -