Three-dimensional growth of glial cell lines affects growth factor and growth factor receptor mRNA levels

Gro Oddveig Ness*, Paal Henning Pedersen, Rolf Bjerkvig, Ole Didrik Laerum, Johan R. Lillehaug

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

We have studied, at the mRNA level, the influence of various defined growth conditions on the expression of TGF-α, PDGF-BB, EGF-R, PDGF-Rα, and PDGF-Rβ in five different glioma cell lines (D-37MG, 54MG, D-263MG, GaMG, and U-251MG). RNA isolated from logarithmically growing, confluent monolayer cells or multicellular spheroids was analyzed. Northern blot experiments show that with a few exceptions, specific mRNA steady-state levels were considerably higher in cells grown in a three-dimensional organization relative to cells in the logarithmic growth phase.

Original languageEnglish
Pages (from-to)433-436
Number of pages4
JournalExperimental Cell Research
Volume214
Issue number1
DOIs
Publication statusPublished - Sep 1994
Externally publishedYes

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