Therapeutic targeting of stat3 using lipopolyplex nanoparticle-formulated sirna in a syngeneic orthotopic mouse glioma model

Benedikt Linder, Ulrike Weirauch, Alexander Ewe, Anja Uhmann, Volker Seifert, Michel Mittelbronn, Patrick N. Harter, Achim Aigner, Donat Kögel*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Glioblastoma (GBM), WHO grade IV, is the most aggressive primary brain tumor in adults. The median survival time using standard therapy is only 12–15 months with a 5-year survival rate of around 5%. Thus, new and effective treatment modalities are of significant importance. Signal transducer and activator of transcription 3 (Stat3) is a key signaling protein driving major hallmarks of cancer and represents a promising target for the development of targeted glioblastoma therapies. Here we present data showing that the therapeutic application of siRNAs, formulated in nanoscale lipopolyplexes (LPP) based on polyethylenimine (PEI) and the phospholipid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), represents a promising new approach to target Stat3 in glioma. We demonstrate that the LPP-mediated delivery of siRNA mediates efficient knockdown of Stat3, suppresses Stat3 activity and limits cell growth in murine (Tu2449) and human (U87, Mz18) glioma cells in vitro. In a therapeutic setting, intracranial application of the siRNA-containing LPP leads to knockdown of STAT3 target gene expression, decreased tumor growth and significantly prolonged survival in Tu2449 glioma-bearing mice compared to negative control-treated animals. This is a proof-of-concept study introducing PEI-based lipopolyplexes as an efficient strategy for therapeutically targeting oncoproteins with otherwise limited druggability

Original languageEnglish
Article number333
JournalCancers
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

Keywords

  • Glioblastoma
  • Glioma
  • Lipopolyplex
  • PEI
  • Polyethylenimine
  • STAT3
  • SiRNA delivery
  • SiRNA/RNAi

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