Abstract
Formation of the CD95 (APO-1/Fas) death inducing signaling complex (DISC) plays a central role in CD95 signaling. Previously, CD95 DISC composition was analyzed by two-dimensional gel electrophoresis and four major cytotoxicity-associated proteins (CAP1/4) were found. CAP1 and CAP2 were defined to be unmodified and phosphorylated FADD, respectively. CAP4 was identified as procaspase-8a. CAP3, however, has remained elusive. In this study, we demonstrate that CAP3 is an intermediate of procaspase-8 processing. CAP3 is generated within seconds of DISC formation and subsequently processed to the prodomain of procaspase-8a that is known as p26 (CAP5). These findings lead to new insights into the mechanism of procaspase-8 processing and apoptosis initiation.
Original language | English |
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Pages (from-to) | 489-498 |
Number of pages | 10 |
Journal | Cell Death and Differentiation |
Volume | 13 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2006 |
Externally published | Yes |
Keywords
- Apoptosis
- CAP3
- CD95