TY - JOUR
T1 - The relation of HPV infection and expression of p53 and p16 proteins in esophageal squamous cells carcinoma
AU - Aguiar Pastrez, Paula Roberta
AU - Mariano, Vânia Sammartino
AU - Costa, Allini Mafra
AU - Silva, Estela Maria
AU - Scapulatempo-Neto, Cristovam
AU - Guimarães, Denise Peixoto
AU - Fava, Gilberto
AU - Neto, Said Abdala Zemi
AU - Nunes, Emily Montosa
AU - Sichero, Laura
AU - Villa, Luisa Lina
AU - Syrjanen, Kari Juhani
AU - Longatto-Filho, Adhemar
N1 - Publisher Copyright:
© Ivyspring International Publisher.
PY - 2017/4/9
Y1 - 2017/4/9
N2 - GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.
AB - GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.
KW - Esophageal neoplasias
KW - HPV
KW - Immunohistochemistry
KW - P16
KW - P53
UR - http://www.scopus.com/inward/record.url?scp=85017622323&partnerID=8YFLogxK
U2 - 10.7150/jca.17080
DO - 10.7150/jca.17080
M3 - Article
AN - SCOPUS:85017622323
SN - 1837-9664
VL - 8
SP - 1062
EP - 1070
JO - Journal of Cancer
JF - Journal of Cancer
IS - 6
ER -