The relation of HPV infection and expression of p53 and p16 proteins in esophageal squamous cells carcinoma

Paula Roberta Aguiar Pastrez, Vânia Sammartino Mariano, Allini Mafra Costa, Estela Maria Silva, Cristovam Scapulatempo-Neto, Denise Peixoto Guimarães, Gilberto Fava, Said Abdala Zemi Neto, Emily Montosa Nunes, Laura Sichero, Luisa Lina Villa, Kari Juhani Syrjanen, Adhemar Longatto-Filho*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC). RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status. CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.

Original languageEnglish
Pages (from-to)1062-1070
Number of pages9
JournalJournal of Cancer
Volume8
Issue number6
DOIs
Publication statusPublished - 9 Apr 2017
Externally publishedYes

Keywords

  • Esophageal neoplasias
  • HPV
  • Immunohistochemistry
  • P16
  • P53

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