The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

Hi Chul Kim; Gi Hwan Kim; David Shum; Ssang Goo Cho; Eun Ju Lee; Yong Jun Kwon

doi:10.1016/j.dib.2016.03.040

The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

Hi Chul Kim
, Gi Hwan Kim
, David Shum
, Ssang Goo Cho
, Eun Ju Lee^*
, Yong Jun Kwon

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, "Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells" (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.

Original language	English
Pages (from-to)	673-678
Number of pages	6
Journal	Data in Brief
Volume	7
DOIs	https://doi.org/10.1016/j.dib.2016.03.040
Publication status	Published - 1 Jun 2016
Externally published	Yes

Access to Document

10.1016/j.dib.2016.03.040

Cite this

@article{60fb00f2ea6949e195073253679c90ab,

title = "The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells",

abstract = "Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, {"}Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells{"} (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.",

author = "Kim, \{Hi Chul\} and Kim, \{Gi Hwan\} and David Shum and Cho, \{Ssang Goo\} and Lee, \{Eun Ju\} and Kwon, \{Yong Jun\}",

note = "Funding Information: This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; Grant nos. NRF-2014K1A4A7A01074642 ), Gyeonggi-do , KISTI , and the Korean Health Technology R\&D Project, Ministry of Health \& Welfare, Republic of Korea (Grant no. HI12C1804 ) Publisher Copyright: {\textcopyright} 2016 The Authors.",

year = "2016",

month = jun,

day = "1",

doi = "10.1016/j.dib.2016.03.040",

language = "English",

volume = "7",

pages = "673--678",

journal = "Data in Brief",

issn = "2352-3409",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

AU - Kim, Hi Chul

AU - Kim, Gi Hwan

AU - Shum, David

AU - Cho, Ssang Goo

AU - Lee, Eun Ju

AU - Kwon, Yong Jun

N1 - Funding Information: This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; Grant nos. NRF-2014K1A4A7A01074642 ), Gyeonggi-do , KISTI , and the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Grant no. HI12C1804 ) Publisher Copyright: © 2016 The Authors.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, "Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells" (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.

AB - Here, we developed a cell defined siRNA microarray (CDSM) for human bone marrow stromal cells (hBMSCs) designed to control the culture of cells inside the spot area without reducing the efficiency of siRNA silencing, "Development of a cell-defined siRNA microarray for analysis of gene functionin human bone marrow stromal cells" (Kim et al., 2016 [1]). First, we confirmed that p65 protein inhibition efficiency was maintained when hBMSCs were culture for 7 days on the siRNA spot, and siRNA spot activity remained in spite of long term storage (10 days and 2 months). Additionally, we confirmed p65 protein inhibition in U2OS cells after 48 h reverse transfection.

UR - https://www.scopus.com/pages/publications/84960871836

UR - https://pubmed.ncbi.nlm.nih.gov/27054175

U2 - 10.1016/j.dib.2016.03.040

DO - 10.1016/j.dib.2016.03.040

M3 - Article

AN - SCOPUS:84960871836

SN - 2352-3409

VL - 7

SP - 673

EP - 678

JO - Data in Brief

JF - Data in Brief

ER -

The monitoring of gene functions on a cell-defined siRNA microarray in human bone marrow stromal and U2OS cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this