TY - JOUR
T1 - The leukocyte activation receptor CD69 controls T cell differentiation through its interaction with galectin-1
AU - de la Fuente, Hortensia
AU - Cruz-Adalia, Aranzazu
AU - del Hoyo, Gloria Martinez
AU - Cibrián-Vera, Danay
AU - Bonay, Pedro
AU - Pérez-Hernández, Daniel
AU - Vázquez, Jesús
AU - Navarro, Pilar
AU - Gutierrez-Gallego, Ricardo
AU - Ramirez-Huesca, Marta
AU - Martín, Pilar
AU - Sánchez-Madrid, Francisco
PY - 2014/7
Y1 - 2014/7
N2 - CD69 is involved in immune cell homeostasis, regulating the T cell-mediated immune response through the control of Th17 cell differentiation. However, natural ligands for CD69 have not yet been described. Using recombinant fusion proteins containing the extracellular domain of CD69, we have detected the presence of a ligand(s) for CD69 on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses of CD69-binding moieties on DCs identified galectin-1 as a CD69 counterreceptor. Surface plasmon resonance and anti-CD69 blocking analyses demonstrated a direct and specific interaction between CD69 and galectin-1 that was carbohydrate dependent. Functional assays with both human and mouse T cells demonstrated the role of CD69 in the negative effect of galectin-1 on Th17 differentiation. Our findings identify CD69 and galectin-1 to be a novel regulatory receptor-ligand pair that modulates Th17 effector cell differentiation and function.
AB - CD69 is involved in immune cell homeostasis, regulating the T cell-mediated immune response through the control of Th17 cell differentiation. However, natural ligands for CD69 have not yet been described. Using recombinant fusion proteins containing the extracellular domain of CD69, we have detected the presence of a ligand(s) for CD69 on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses of CD69-binding moieties on DCs identified galectin-1 as a CD69 counterreceptor. Surface plasmon resonance and anti-CD69 blocking analyses demonstrated a direct and specific interaction between CD69 and galectin-1 that was carbohydrate dependent. Functional assays with both human and mouse T cells demonstrated the role of CD69 in the negative effect of galectin-1 on Th17 differentiation. Our findings identify CD69 and galectin-1 to be a novel regulatory receptor-ligand pair that modulates Th17 effector cell differentiation and function.
UR - http://www.scopus.com/inward/record.url?scp=84901813814&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/24752896
U2 - 10.1128/MCB.00348-14
DO - 10.1128/MCB.00348-14
M3 - Article
C2 - 24752896
AN - SCOPUS:84901813814
SN - 0270-7306
VL - 34
SP - 2479
EP - 2487
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 13
ER -