The invasive proteome of glioblastoma revealed by laser-capture microdissection

Thomas Daubon; Joris Guyon; Anne Aurélie Raymond; Benjamin Dartigues; Justine Rudewicz; Zakaria Ezzoukhry; Jean William Dupuy; John M.J. Herbert; Frédéric Saltel; Rolf Bjerkvig; Macha Nikolski; Andreas Bikfalvi

doi:10.1093/noajnl/vdz029

The invasive proteome of glioblastoma revealed by laser-capture microdissection

Thomas Daubon
, Joris Guyon
, Anne Aurélie Raymond
, Benjamin Dartigues
, Justine Rudewicz
, Zakaria Ezzoukhry
, Jean William Dupuy
, John M.J. Herbert
, Frédéric Saltel
, Rolf Bjerkvig
, Macha Nikolski
, Andreas Bikfalvi^*

^*Corresponding author for this work

Department of Cancer Research

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

Background: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.

Methods: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.

Results: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.

Conclusions: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

Original language	English
Article number	vdz029
Journal	Neuro-Oncology Advances
Volume	1
Issue number	1
DOIs	https://doi.org/10.1093/noajnl/vdz029
Publication status	Published - May 2019

Keywords

glioblastoma
intratumor heterogeneity
invasion
patient-derived xenograft
proteomics analysis

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10.1093/noajnl/vdz029Licence: CC BY

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title = "The invasive proteome of glioblastoma revealed by laser-capture microdissection",

abstract = "Background: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.Methods: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.Results: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.Conclusions: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.",

keywords = "glioblastoma, intratumor heterogeneity, invasion, patient-derived xenograft, proteomics analysis",

author = "Thomas Daubon and Joris Guyon and Raymond, \{Anne Aur{\'e}lie\} and Benjamin Dartigues and Justine Rudewicz and Zakaria Ezzoukhry and Dupuy, \{Jean William\} and Herbert, \{John M.J.\} and Fr{\'e}d{\'e}ric Saltel and Rolf Bjerkvig and Macha Nikolski and Andreas Bikfalvi",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s) 2019. Published by Oxford University Press, the Society for Neuro-Oncology and the European Association of Neuro-Oncology.",

year = "2019",

month = may,

doi = "10.1093/noajnl/vdz029",

language = "English",

volume = "1",

journal = "Neuro-Oncology Advances",

issn = "2632-2498",

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TY - JOUR

T1 - The invasive proteome of glioblastoma revealed by laser-capture microdissection

AU - Daubon, Thomas

AU - Guyon, Joris

AU - Raymond, Anne Aurélie

AU - Dartigues, Benjamin

AU - Rudewicz, Justine

AU - Ezzoukhry, Zakaria

AU - Dupuy, Jean William

AU - Herbert, John M.J.

AU - Saltel, Frédéric

AU - Bjerkvig, Rolf

AU - Nikolski, Macha

AU - Bikfalvi, Andreas

PY - 2019/5

Y1 - 2019/5

N2 - Background: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.Methods: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.Results: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.Conclusions: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

AB - Background: Glioblastomas are heterogeneous tumors composed of a necrotic and tumor core and an invasive periphery.Methods: Here, we performed a proteomics analysis of laser-capture micro-dissected glioblastoma core and invasive areas of patient-derived xenografts.Results: Bioinformatics analysis identified enriched proteins in central and invasive tumor areas. Novel markers of invasion were identified, the genes proteolipid protein 1 (PLP1) and Dynamin-1 (DNM1), which were subsequently validated in tumors and by functional assays.Conclusions: In summary, our results identify new networks and molecules that may play an important role in glioblastoma development and may constitute potential novel therapeutic targets.

KW - glioblastoma

KW - intratumor heterogeneity

KW - invasion

KW - patient-derived xenograft

KW - proteomics analysis

UR - https://www.scopus.com/pages/publications/85085518575

UR - https://pubmed.ncbi.nlm.nih.gov/32642662

U2 - 10.1093/noajnl/vdz029

DO - 10.1093/noajnl/vdz029

M3 - Article

C2 - 32642662

SN - 2632-2498

VL - 1

JO - Neuro-Oncology Advances

JF - Neuro-Oncology Advances

IS - 1

M1 - vdz029

ER -

The invasive proteome of glioblastoma revealed by laser-capture microdissection

Abstract

Keywords

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