The Immunological Synapse Part B

Clément Thomas (Editor), Lorenzo Galluzzi (Editor)

Research output: Book/ReportBookpeer-review


he Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay.
Original languageEnglish
PublisherAcademic Press Inc.
Number of pages318
ISBN (Electronic)978-0-443-19164-0
ISBN (Print)978-0-443-19163-3
Publication statusPublished - 11 Jul 2023

Publication series

NameMethods in Cell Biology
PublisherAcademic Press
ISSN (Print)0091-679X
ISSN (Electronic)0091-679X


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