TY - JOUR
T1 - The Identification of Enteric Fever-Specific Antigens for Population-Based Serosurveillance
AU - Mylona, Elli
AU - Hefele, Lisa
AU - Thieu, Nga Tran Vu
AU - Van, Tan Trinh
AU - Minh, Chau Nguyen Ngoc
AU - Tuan, Anh Tran
AU - Karkey, Abhilasha
AU - Dongol, Sabina
AU - Basnyat, Buddha
AU - Vinh, Phat Voong
AU - Dan, Thanh Ho Ngoc
AU - Russell, Paula
AU - Charles, Richelle C.
AU - Parry, Christopher M.
AU - Baker, Stephen
N1 - Publisher Copyright:
© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America.
PY - 2024/3/15
Y1 - 2024/3/15
N2 - BACKGROUND: Enteric fever, caused by Salmonella enterica serovars Typhi and Paratyphi A, is a major public health problem in low and middle-income countries. Moderate sensitivity and scalability of current methods likely underestimate enteric fever burden. Determining the serological responses to organism-specific antigens may improve incidence measures.METHODS: Plasma samples were collected from blood culture-confirmed enteric fever patients, blood culture-negative febrile patients over the course of three months and afebrile community controls. A panel of 17 Salmonella Typhi and Paratyphi A antigens was purified and used to determine antigen-specific antibody responses by indirect ELISAs.RESULTS: The antigen-specific longitudinal antibody responses were comparable between enteric fever patients, patients with blood culture-negative febrile controls, and afebrile community controls for most antigens. However, we found that IgG responses against STY1479 (YncE), STY1886 (CdtB), STY1498 (HlyE) and the serovar-specific O2 and O9 antigens were greatly elevated over a three-month follow up period in S. Typhi/S. Paratyphi A patients compared to controls, suggesting seroconversion.CONCLUSIONS: We identified a set of antigens as good candidates to demonstrate enteric fever exposure. These targets can be used in combination to develop more sensitive and scalable approaches to enteric fever surveillance and generate invaluable epidemiological data for informing vaccine policies.
AB - BACKGROUND: Enteric fever, caused by Salmonella enterica serovars Typhi and Paratyphi A, is a major public health problem in low and middle-income countries. Moderate sensitivity and scalability of current methods likely underestimate enteric fever burden. Determining the serological responses to organism-specific antigens may improve incidence measures.METHODS: Plasma samples were collected from blood culture-confirmed enteric fever patients, blood culture-negative febrile patients over the course of three months and afebrile community controls. A panel of 17 Salmonella Typhi and Paratyphi A antigens was purified and used to determine antigen-specific antibody responses by indirect ELISAs.RESULTS: The antigen-specific longitudinal antibody responses were comparable between enteric fever patients, patients with blood culture-negative febrile controls, and afebrile community controls for most antigens. However, we found that IgG responses against STY1479 (YncE), STY1886 (CdtB), STY1498 (HlyE) and the serovar-specific O2 and O9 antigens were greatly elevated over a three-month follow up period in S. Typhi/S. Paratyphi A patients compared to controls, suggesting seroconversion.CONCLUSIONS: We identified a set of antigens as good candidates to demonstrate enteric fever exposure. These targets can be used in combination to develop more sensitive and scalable approaches to enteric fever surveillance and generate invaluable epidemiological data for informing vaccine policies.
KW - enteric fever
KW - IgG antibodies
KW - longitudinal responses
KW - serosurveillance
UR - http://www.scopus.com/inward/record.url?scp=85179704770&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/37403670
U2 - 10.1093/infdis/jiad242
DO - 10.1093/infdis/jiad242
M3 - Article
C2 - 37403670
SN - 0022-1899
VL - 229
SP - 833
EP - 844
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 3
ER -