TY - JOUR
T1 - The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila
AU - Deddouche, Safia
AU - Matt, Nicolas
AU - Budd, Aidan
AU - Mueller, Stefanie
AU - Kemp, Cordula
AU - Galiana-Arnoux, Delphine
AU - Dostert, Catherine
AU - Antoniewski, Christophe
AU - Hoffmann, Jules A.
AU - Imler, Jean Luc
N1 - Funding Information:
We thank E. Santiago and S. Ozkan for technical expertise; R. Carthew (Northwestern University), J.-M. Reichhart (Unité Propre de Recherché 9022 Centre National de la Recherche Scientifique) and M. Siomi (University of Tokushima) for fly stocks; A. Schneeman (The Scripps Research Institute) for FHV and anti-FHV; D. Ferrandon for critical reading of the manuscript and comments; and E. Levashina for discussions. Confocal microscopy was done at the Strasbourg Esplanade Cellular Imaging Facility (funded by the Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Louis Pasteur University and Alsace Region). Supported by the US National Institutes of Health (PO1 AI070167), Agence Nationale de Recherche Microbiologie-Maladie Emergentes and Centre National de la Recherche Scientifique.
PY - 2008
Y1 - 2008
N2 - Drosophila, like other invertebrates and plants, relies mainly on RNA interference for its defense against viruses. In flies, viral infection also triggers the expression of many genes. One of the genes induced, Vago, encodes a 18-kilodalton cysteine-rich polypeptide. Here we provide genetic evidence that the Vago gene product controlled viral load in the fat body after infection with drosophila C virus. Induction of Vago was dependent on the helicase Dicer-2. Dicer-2 belongs to the same DExD/ H-box helicase family as do the RIG-I-like receptors, which sense viral infection and mediate interferon induction in mammals. We propose that this family represents an evolutionary conserved set of sensors that detect viral nucleic acids and direct antiviral responses.
AB - Drosophila, like other invertebrates and plants, relies mainly on RNA interference for its defense against viruses. In flies, viral infection also triggers the expression of many genes. One of the genes induced, Vago, encodes a 18-kilodalton cysteine-rich polypeptide. Here we provide genetic evidence that the Vago gene product controlled viral load in the fat body after infection with drosophila C virus. Induction of Vago was dependent on the helicase Dicer-2. Dicer-2 belongs to the same DExD/ H-box helicase family as do the RIG-I-like receptors, which sense viral infection and mediate interferon induction in mammals. We propose that this family represents an evolutionary conserved set of sensors that detect viral nucleic acids and direct antiviral responses.
UR - http://www.scopus.com/inward/record.url?scp=56349115840&partnerID=8YFLogxK
U2 - 10.1038/ni.1664
DO - 10.1038/ni.1664
M3 - Article
C2 - 18953338
AN - SCOPUS:56349115840
SN - 1529-2908
VL - 9
SP - 1425
EP - 1432
JO - Nature Immunology
JF - Nature Immunology
IS - 12
ER -