The acute and temporary modulation of PERIOD genes by hydrocortisone in healthy subjects

Türkan Yurtsever*, Thomas M. Schilling, Monika Kölsch, Jonathan D. Turner, Jobst Meyer, Hartmut Schächinger, Andrea B. Schote

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)


The physiological stress system and the circadian clock system communicate with each other at different signaling levels. The steroid hormone cortisol, the end-effector of the hypothalamus–pituitary–adrenal axis, is released in response to stress and acts as a mediator in circadian rhythms. We determined the effect of escalating cortisol doses on the expression of PERIOD genes (PER1, PER2 and PER3) in healthy subjects and analyzed whether the glucocorticoid receptor (GR) is involved in the cortisol-mediated PERIOD gene expression. Forty participants (50% males and 50% females) were randomly assigned to groups receiving a saline placebo solution or 3 mg, 6 mg, 12 mg and 24 mg of hydrocortisone. Blood was drawn every 15 min to measure quantitative gene expression of PER1, PER2 and PER3. A potential role of the GR was determined by an ex vivo study stimulating whole blood with hydrocortisone and RU486 (a GR antagonist). As a result, moderate doses of hydrocortisone produced an acute and temporary induction of PER1 and PER3 mRNA levels, whereas PER2 was not responsive to the hormone administration. The cortisol-dependent induction of PER1 was blocked by the GR antagonist in whole blood after treatment with hydrocortisone and RU486 ex vivo. In conclusion, acute pharmacological stress modulated the expression of PER1 and PER3 in whole blood temporarily in our short-term sampling design, suggesting that these circadian genes mediate stable molecular mechanisms in the periphery.

Original languageEnglish
Pages (from-to)1222-1234
Number of pages13
JournalChronobiology International
Issue number9
Publication statusPublished - 20 Oct 2016


  • HPA axis
  • PERIOD genes
  • circadian clock
  • healthy subjects
  • hydrocortisone


Dive into the research topics of 'The acute and temporary modulation of PERIOD genes by hydrocortisone in healthy subjects'. Together they form a unique fingerprint.

Cite this