TDCIPP exposure affects Artemia franciscana growth and osmoregulation

Melissa A. Morgan, Corey M. Griffith, David C. Volz, Cynthia K. Larive*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Environmental monitoring has demonstrated widespread occurrence of the flame-retardant tris(1,3-dichloro-2-propyl) phosphate (TDCIPP), raising concerns about the impact on aquatic life. Using 1H NMR and GC–MS metabolomics and 20-day body length experiments, we have determined that exposure to TDCIPP affects Artemia franciscana. The LC50 for a 48 h TDCIPP exposure was determined to be 37.1 ± 1.3 μM. Acute exposure (48 h) to 20.0 μM did not affect A. franciscana body length but did elicit a metabolic change. Chronic exposure to 0.50 μM TDCIPP caused decreased body length in A. franciscana exposed for 20 days and elicited a metabolic response. Principal component analysis revealed variance between acute and chronic exposure along PC1 (36.4%) and between control and TDCIPP along PC2 (17.4%). One-way ANOVA indicated that 19 metabolites were significantly affected by TDCIPP exposure; namely metabolites of the osmolyte class, including betaine, phosphocholine, gadusol, taurine, glycerol and trehalose – metabolites that are essential osmoprotectants in extremophile species. Other pathways that may be perturbed by TDCIPP exposure include one carbon, glycine, serine, threonine, and glycerophospholipid metabolism.

Original languageEnglish
Article number133486
JournalScience of the Total Environment
Volume694
DOIs
Publication statusPublished - 1 Dec 2019
Externally publishedYes

Keywords

  • H NMR
  • Artemia franciscana
  • GC–MS
  • Metabolomics
  • Osmoregulation
  • Tris(1,3-dichloro-2-propyl) phosphate

Fingerprint

Dive into the research topics of 'TDCIPP exposure affects Artemia franciscana growth and osmoregulation'. Together they form a unique fingerprint.

Cite this