Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates

Wilhelm J. Richter; Dieter R. Müller; Bruno Domon

doi:10.1016/0076-6879(90)93441-M

Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates

Wilhelm J. Richter
, Dieter R. Müller
, Bruno Domon

Research output: Contribution to journal › Article › Research › peer-review

35 Citations (Scopus)

Abstract

This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.

Original language	English
Pages (from-to)	607-623
Number of pages	17
Journal	Methods in Enzymology
Volume	193
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(90)93441-M
Publication status	Published - 1 Jan 1990
Externally published	Yes

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title = "Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates",

abstract = "This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.",

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AU - Domon, Bruno

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N2 - This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.

AB - This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.

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DO - 10.1016/0076-6879(90)93441-M

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JO - Methods in Enzymology

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Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates

Abstract

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