TY - JOUR
T1 - Tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates
AU - Richter, Wilhelm J.
AU - Müller, Dieter R.
AU - Domon, Bruno
PY - 1990/1/1
Y1 - 1990/1/1
N2 - This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.
AB - This chapter discusses tandem mass spectrometry in structural characterization of oligosaccharide residues in glycoconjugates. In tandem mass spectrometry (MS/MS), two-stage mass analysis is performed. In the first stage (MS-l), a specific ion of interest is selected according to its mass from the set of ions that constitute the conventional mass spectrum. The second stage (MS-2) generates, from this selected precursor ion, a spectrum of product ions that arise from metastable ion (MI) or collision-induced dissociation (CID). The discussion in this chapter is limited to the structural analysis of small carbohydrate subunits (up to 5 sugar units) attached to larger aglycon moieties. At present, MS/MS of sugar ions formed from glycoconjugates appears to fulfill some, but not all, of the basic requirements of structure analysis.
UR - http://www.scopus.com/inward/record.url?scp=0025637286&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(90)93441-M
DO - 10.1016/0076-6879(90)93441-M
M3 - Article
C2 - 2074838
AN - SCOPUS:0025637286
SN - 0076-6879
VL - 193
SP - 607
EP - 623
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -