Systemic first-line phenotyping

Valérie Gailus-Durner*, Helmut Fuchs, Thure Adler, Antonio Aguilar Pimentel, Lore Becker, Ines Bolle, Julia Calzada-Wack, Claudia Dalke, Nicole Ehrhardt, Barbara Ferwagner, Wolfgang Hans, Sabine M. Hölter, Gabriele Hölzlwimmer, Marion Horsch, Anahita Javaheri, Magdalena Kallnik, Eva Kling, Christoph Lengger, Corinna Mörth, Ilona MossbruggerBeatrix Naton, Cornelia Prehn, Oliver Puk, Birgit Rathkolb, Jan Rozman, Anja Schrewe, Frank Thiele, Jerzy Adamski, Bernhard Aigner, Heidrun Behrendt, Dirk H. Busch, Jack Favor, Jochen Graw, Gerhard Heldmaier, Boris Ivandic, Hugo Katus, Martin Klingenspor, Thomas Klopstock Elisabeth Kremmer, Markus Ollert, Leticia Quintanilla-Martinez, Holger Schulz, Eckhard Wolf, Wolfgang Wurst, Martin Hrabé De Angelis

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

66 Citations (Scopus)

Abstract

∈With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.

Original languageEnglish
Title of host publicationGene Knockout Protocols
Subtitle of host publicationSecond Edition
EditorsRalf Kuhn, Wolfgang Wurst
Pages463-509
Number of pages47
DOIs
Publication statusPublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume530
ISSN (Print)1064-3745

Keywords

  • IgE
  • Mouse clinic
  • airway inflammation
  • allergy
  • antibody
  • asthma
  • atrial natriuretic peptide
  • bead-array
  • blood
  • blood pressure
  • bone metabolism
  • bronchoalveolar lavage
  • cachexia
  • cardiovascular disease
  • clinical chemistry
  • diabetes energy expenditure
  • energy intake
  • eye
  • flow cytometry
  • hematology
  • infection
  • lung function
  • lymphocyte
  • motor coordination
  • muscle function
  • neurological disease
  • obesity
  • osteoporosis
  • pathology
  • phenotyping
  • skeletomuscular diseases
  • steroid metabolism
  • urine

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