Systemic first-line phenotyping

Valérie Gailus-Durner; Helmut Fuchs; Thure Adler; Antonio Aguilar Pimentel; Lore Becker; Ines Bolle; Julia Calzada-Wack; Claudia Dalke; Nicole Ehrhardt; Barbara Ferwagner; Wolfgang Hans; Sabine M. Hölter; Gabriele Hölzlwimmer; Marion Horsch; Anahita Javaheri; Magdalena Kallnik; Eva Kling; Christoph Lengger; Corinna Mörth; Ilona Mossbrugger; Beatrix Naton; Cornelia Prehn; Oliver Puk; Birgit Rathkolb; Jan Rozman; Anja Schrewe; Frank Thiele; Jerzy Adamski; Bernhard Aigner; Heidrun Behrendt; Dirk H. Busch; Jack Favor; Jochen Graw; Gerhard Heldmaier; Boris Ivandic; Hugo Katus; Martin Klingenspor; Thomas Klopstock Elisabeth Kremmer; Markus Ollert; Leticia Quintanilla-Martinez; Holger Schulz; Eckhard Wolf; Wolfgang Wurst; Martin Hrabé De Angelis

doi:10.1007/978-1-59745-471-1_25

Systemic first-line phenotyping

Valérie Gailus-Durner^*, Helmut Fuchs, Thure Adler, Antonio Aguilar Pimentel, Lore Becker, Ines Bolle, Julia Calzada-Wack, Claudia Dalke, Nicole Ehrhardt, Barbara Ferwagner, Wolfgang Hans, Sabine M. Hölter, Gabriele Hölzlwimmer, Marion Horsch, Anahita Javaheri, Magdalena Kallnik, Eva Kling, Christoph Lengger, Corinna Mörth, Ilona MossbruggerBeatrix Naton, Cornelia Prehn, Oliver Puk, Birgit Rathkolb, Jan Rozman, Anja Schrewe, Frank Thiele, Jerzy Adamski, Bernhard Aigner, Heidrun Behrendt, Dirk H. Busch, Jack Favor, Jochen Graw, Gerhard Heldmaier, Boris Ivandic, Hugo Katus, Martin Klingenspor, Thomas Klopstock Elisabeth Kremmer, Markus Ollert, Leticia Quintanilla-Martinez, Holger Schulz, Eckhard Wolf, Wolfgang Wurst, Martin Hrabé De Angelis

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

68 Citations (Scopus)

Abstract

∈With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.

Original language	English
Title of host publication	Gene Knockout Protocols
Subtitle of host publication	Second Edition
Editors	Ralf Kuhn, Wolfgang Wurst
Pages	463-509
Number of pages	47
DOIs	https://doi.org/10.1007/978-1-59745-471-1_25
Publication status	Published - 2009
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	530
ISSN (Print)	1064-3745

Keywords

IgE
Mouse clinic
airway inflammation
allergy
antibody
asthma
atrial natriuretic peptide
bead-array
blood
blood pressure
bone metabolism
bronchoalveolar lavage
cachexia
cardiovascular disease
clinical chemistry
diabetes energy expenditure
energy intake
eye
flow cytometry
hematology
infection
lung function
lymphocyte
motor coordination
muscle function
neurological disease
obesity
osteoporosis
pathology
phenotyping
skeletomuscular diseases
steroid metabolism
urine

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10.1007/978-1-59745-471-1_25

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Gailus-Durner, V., Fuchs, H., Adler, T., Aguilar Pimentel, A., Becker, L., Bolle, I., Calzada-Wack, J., Dalke, C., Ehrhardt, N., Ferwagner, B., Hans, W., Hölter, S. M., Hölzlwimmer, G., Horsch, M., Javaheri, A., Kallnik, M., Kling, E., Lengger, C., Mörth, C., ... De Angelis, M. H. (2009). Systemic first-line phenotyping. In R. Kuhn, & W. Wurst (Eds.), Gene Knockout Protocols: Second Edition (pp. 463-509). (Methods in Molecular Biology; Vol. 530). https://doi.org/10.1007/978-1-59745-471-1_25

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abstract = "∈With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.",

keywords = "IgE, Mouse clinic, airway inflammation, allergy, antibody, asthma, atrial natriuretic peptide, bead-array, blood, blood pressure, bone metabolism, bronchoalveolar lavage, cachexia, cardiovascular disease, clinical chemistry, diabetes energy expenditure, energy intake, eye, flow cytometry, hematology, infection, lung function, lymphocyte, motor coordination, muscle function, neurological disease, obesity, osteoporosis, pathology, phenotyping, skeletomuscular diseases, steroid metabolism, urine",

author = "Val{\'e}rie Gailus-Durner and Helmut Fuchs and Thure Adler and {Aguilar Pimentel}, Antonio and Lore Becker and Ines Bolle and Julia Calzada-Wack and Claudia Dalke and Nicole Ehrhardt and Barbara Ferwagner and Wolfgang Hans and H{\"o}lter, {Sabine M.} and Gabriele H{\"o}lzlwimmer and Marion Horsch and Anahita Javaheri and Magdalena Kallnik and Eva Kling and Christoph Lengger and Corinna M{\"o}rth and Ilona Mossbrugger and Beatrix Naton and Cornelia Prehn and Oliver Puk and Birgit Rathkolb and Jan Rozman and Anja Schrewe and Frank Thiele and Jerzy Adamski and Bernhard Aigner and Heidrun Behrendt and Busch, {Dirk H.} and Jack Favor and Jochen Graw and Gerhard Heldmaier and Boris Ivandic and Hugo Katus and Martin Klingenspor and {Elisabeth Kremmer}, {Thomas Klopstock} and Markus Ollert and Leticia Quintanilla-Martinez and Holger Schulz and Eckhard Wolf and Wolfgang Wurst and {De Angelis}, {Martin Hrab{\'e}}",

year = "2009",

doi = "10.1007/978-1-59745-471-1_25",

language = "English",

isbn = "9781934115268",

series = "Methods in Molecular Biology",

pages = "463--509",

editor = "Ralf Kuhn and Wolfgang Wurst",

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Gailus-Durner, V, Fuchs, H, Adler, T, Aguilar Pimentel, A, Becker, L, Bolle, I, Calzada-Wack, J, Dalke, C, Ehrhardt, N, Ferwagner, B, Hans, W, Hölter, SM, Hölzlwimmer, G, Horsch, M, Javaheri, A, Kallnik, M, Kling, E, Lengger, C, Mörth, C, Mossbrugger, I, Naton, B, Prehn, C, Puk, O, Rathkolb, B, Rozman, J, Schrewe, A, Thiele, F, Adamski, J, Aigner, B, Behrendt, H, Busch, DH, Favor, J, Graw, J, Heldmaier, G, Ivandic, B, Katus, H, Klingenspor, M, Elisabeth Kremmer, TK, Ollert, M, Quintanilla-Martinez, L, Schulz, H, Wolf, E, Wurst, W & De Angelis, MH 2009, Systemic first-line phenotyping. in R Kuhn & W Wurst (eds), Gene Knockout Protocols: Second Edition. Methods in Molecular Biology, vol. 530, pp. 463-509. https://doi.org/10.1007/978-1-59745-471-1_25

TY - CHAP

T1 - Systemic first-line phenotyping

AU - Gailus-Durner, Valérie

AU - Fuchs, Helmut

AU - Adler, Thure

AU - Aguilar Pimentel, Antonio

AU - Becker, Lore

AU - Bolle, Ines

AU - Calzada-Wack, Julia

AU - Dalke, Claudia

AU - Ehrhardt, Nicole

AU - Ferwagner, Barbara

AU - Hans, Wolfgang

AU - Hölter, Sabine M.

AU - Hölzlwimmer, Gabriele

AU - Horsch, Marion

AU - Javaheri, Anahita

AU - Kallnik, Magdalena

AU - Kling, Eva

AU - Lengger, Christoph

AU - Mörth, Corinna

AU - Mossbrugger, Ilona

AU - Naton, Beatrix

AU - Prehn, Cornelia

AU - Puk, Oliver

AU - Rathkolb, Birgit

AU - Rozman, Jan

AU - Schrewe, Anja

AU - Thiele, Frank

AU - Adamski, Jerzy

AU - Aigner, Bernhard

AU - Behrendt, Heidrun

AU - Busch, Dirk H.

AU - Favor, Jack

AU - Graw, Jochen

AU - Heldmaier, Gerhard

AU - Ivandic, Boris

AU - Katus, Hugo

AU - Klingenspor, Martin

AU - Elisabeth Kremmer, Thomas Klopstock

AU - Ollert, Markus

AU - Quintanilla-Martinez, Leticia

AU - Schulz, Holger

AU - Wolf, Eckhard

AU - Wurst, Wolfgang

AU - De Angelis, Martin Hrabé

PY - 2009

Y1 - 2009

N2 - ∈With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.

AB - ∈With the completion of the mouse genome sequence an essential task for biomedical sciences in the twenty-first century will be the generation and functional analysis of mouse models for every gene in the mammalian genome. More than 30,000 mutations in ES cells will be engineered and thousands of mouse disease models will become available over the coming years by the collaborative effort of the International Mouse Knockout Consortium. In order to realize the full value of the mouse models proper characterization, archiving and dissemination of mouse disease models to the research community have to be performed. Phenotyping centers (mouse clinics) provide the necessary capacity, broad expertise, equipment, and infrastructure to carry out large-scale systemic first-line phenotyping. Using the example of the German Mouse Clinic (GMC) we will introduce the reader to the different aspects of the organization of a mouse clinic and present selected methods used in first-line phenotyping.

KW - IgE

KW - Mouse clinic

KW - airway inflammation

KW - allergy

KW - antibody

KW - asthma

KW - atrial natriuretic peptide

KW - bead-array

KW - blood

KW - blood pressure

KW - bone metabolism

KW - bronchoalveolar lavage

KW - cachexia

KW - cardiovascular disease

KW - clinical chemistry

KW - diabetes energy expenditure

KW - energy intake

KW - eye

KW - flow cytometry

KW - hematology

KW - infection

KW - lung function

KW - lymphocyte

KW - motor coordination

KW - muscle function

KW - neurological disease

KW - obesity

KW - osteoporosis

KW - pathology

KW - phenotyping

KW - skeletomuscular diseases

KW - steroid metabolism

KW - urine

UR - http://www.scopus.com/inward/record.url?scp=65549149594&partnerID=8YFLogxK

U2 - 10.1007/978-1-59745-471-1_25

DO - 10.1007/978-1-59745-471-1_25

M3 - Chapter

C2 - 19266331

AN - SCOPUS:65549149594

SN - 9781934115268

T3 - Methods in Molecular Biology

SP - 463

EP - 509

BT - Gene Knockout Protocols

A2 - Kuhn, Ralf

A2 - Wurst, Wolfgang

ER -

Systemic first-line phenotyping

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Keywords

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