Staphylococcus aureus fibronectin-binding protein specifically binds IgE from patients with atopic dermatitis and requires antigen presentation for cellular immune responses

Kavita Reginald, Kerstin Westritschnig, Birgit Linhart, Margarete Focke-Tejkl, Beatrice Jahn-Schmid, Julia Eckl-Dorna, Annice Heratizadeh, Angelika Stöcklinger, Nadja Balic, Susanne Spitzauer, Verena Niederberger, Thomas Werfel, Josef Thalhamer, Stephan Weidinger, Natalija Novak, Markus Ollert, Alexander M. Hirschl, Rudolf Valenta*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

35 Citations (Scopus)

Abstract

Background: Staphylococcus aureus superinfections occur in more than 90% of patients with atopic dermatitis (AD) and aggravate skin inflammation. S aureus toxins lead to tissue damage and augment T-cell-mediated skin inflammation by a superantigen effect. Objective: To characterize IgE-reactive proteins from S aureus. Methods: A genomic S aureus library was screened with IgE from patients with AD for DNA clones coding for IgE-reactive antigens. One was identified as fibronectin-binding protein (FBP). Recombinant FBP was expressed in Escherichia coli, purified, and tested for specific IgE reactivity in patients with AD. Its allergenic activity was studied in basophil activation experiments and T-cell cultures. The in vivo allergenic activity was investigated by sensitizing mice. Results: Using IgE from patients with AD for screening of a genomic S aureus library, an IgE-reactive DNA clone was isolated that coded for FBP. Recombinant FBP was expressed in E coli and purified. It reacted specifically with IgE from patients with AD and exhibited allergenic activity in basophil degranulation assays. FBP showed specific T-cell reactivity requiring antigen presentation and induced the secretion of proinflammatory cytokines from PBMCs. Mice sensitized with FBP mounted FBP-specific IgE responses, showed FBP-specific basophil degranulation as well as FBP-specific T-cell proliferation, and mixed T h2/Th1 cytokine secretion. Conclusion: Evidence is provided that specific humoral and cellular immune responses to S aureus antigens dependent on antigen presentation represent a novel mechanism for S aureus-induced skin inflammation in AD. Furthermore, FBP may be used for the development of novel diagnostic and therapeutic strategies for S aureus infections.

Original languageEnglish
Pages (from-to)82-91.e8
JournalJournal of Allergy and Clinical Immunology
Volume128
Issue number1
DOIs
Publication statusPublished - Jul 2011
Externally publishedYes

Keywords

  • IgE
  • Staphylococcus aureus
  • allergy
  • atopic dermatitis
  • bacterial allergen
  • bacterial antigen
  • superantigen
  • superinfection

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