Specific Antibodies Elicited By Antigen Covalently Linked To A Synthetic Adjuvant

W. G. Bessler*, Barbara Suhr, H. J. Bühring, C. P. Muller, K. H. Wiesmüller, G. Becker, G. Jung

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

42 Citations (Scopus)


S-(2,3-Bis(palmitoyloxy)-(2 RS)-propyl)-N-palmitoyl-(R)-cysteinyl-(S)-serine (Tripam-Cys-Ser), a synthetic analogue of the N-terminal part of bacterial lipoprotein, constitutes a potent B-lymphocyte activator, inducing mitosis and immunoglobulin production, and is an adjuvant of the humoral immune response. The product was covalently coupled to a non-immunogenic oligopeptide, representing an extracytoplasmic sequence of the receptor for the epidermal growth factor (EGF-R amino acids 516–529: Asn-Leu-Leu-Glu-Gly-Glu-Pro-Arg-Glu-Phe-Val-Glu-Asn-Ser). The resulting conjugate was able to induce a specific antibody response 2 wk after a single i.p. administration in mice, as measured by ELISA assays. Controls performed by injecting either adjuvant or oligopeptide alone, or a mixture of both compounds, elicited only a marginal stimulation of the specific antibody response. The conjugate was also immunogenic in vitro. Synthetically derived lipopeptides, thus, constitute powerful agents for turning substances of low immunogenicity into potent immunogens, after conjugating them covalently to the adjuvants. This technique should have a wide application in the preparation of conventional and monoclonal antibodies, as well as for the design of synthetic vaccines.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
Issue number3
Publication statusPublished - 1985
Externally publishedYes


  • Amino Acids 516-529 of the EGF-R
  • EGF-R
  • Pep 14
  • Receptor for the epidermal growth factor
  • S-(2,3-Bis(palmitoyloxy)-(2iRS)-propyl)-N-palmitoyl-(R)-cystem-(S)-serine;
  • Tripam-cys-ser


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