Simultaneous extraction of proteins and metabolites from cells in culture

Sean C. Sapcariu*, Tamara Kanashova, Daniel Weindl, Jenny Ghelfi, Gunnar Dittmar, Karsten Hiller

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

98 Citations (Scopus)

Abstract

Proper sample preparation is an integral part of all omics approaches, and can drastically impact the results of a wide number of analyses. As metabolomics and proteomics research approaches often yield complementary information, it is desirable to have a sample preparation procedure which can yield information for both types of analyses from the same cell population. This protocol explains a method for the separation and isolation of metabolites and proteins from the same biological sample, in order for downstream use in metabolomics and proteomics analyses simultaneously. In this way, two different levels of biological regulation can be studied in a single sample, minimizing the variance that would result from multiple experiments. This protocol can be used with both adherent and suspension cell cultures, and the extraction of metabolites from cellular medium is also detailed, so that cellular uptake and secretion of metabolites can be quantified. Advantages of this technique includes:Inexpensive and quick to perform; this method does not require any kits.Can be used on any cells in culture, including cell lines and primary cells extracted from living organisms.A wide variety of different analysis techniques can be used, adding additional value to metabolomics data analyzed from a sample; this is of high value in experimental systems biology.

Original languageEnglish
Pages (from-to)74-80
Number of pages7
JournalMethodsX
Volume1
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Biomolecule extraction
  • GC/MS
  • Isolation
  • LC/MS
  • Metabolomics
  • Proteomics
  • Sample preparation

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