SILAC for biomarker discovery

Gunnar Dittmar, Matthias Selbach*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Citations (Scopus)


SILAC has been employed in MS-based proteomics for nearly a decade. This method is based on cells in culture metabolically incorporating isotope-coded essential amino acids and allows the quantification of global protein populations to identify characteristic changes. Variations of this technique developed over the years allow the application of SILAC not only to cell culture derived samples but also to tissues and human specimens, making this powerful technique amenable to clinically relevant samples. In this review, we provide an overview of different SILAC-derived methods and their use in the identification and development of biomarkers.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalProteomics - Clinical Applications
Issue number3-4
Publication statusPublished - 1 Apr 2015
Externally publishedYes


  • MS
  • Quantification
  • Quantitative proteomics


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