SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase

Kaspar Reier; Aivar Liiv; Jaanus Remme

doi:10.1128/mra.00042-24

SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase

Kaspar Reier^*, Aivar Liiv, Jaanus Remme^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The expression level of individual proteins varies markedly during the progression of the growth phase in bacteria. A set of proteins was quantified in Escherichia coli total proteome during 14 days of batch cultivation using pulse stable isotope labeled amino acids in cell culture (SILAC)-based quantitative mass spectrometry.

Original language	English
Journal	Microbiology Resource Announcements
Volume	13
Issue number	6
DOIs	https://doi.org/10.1128/mra.00042-24
Publication status	Published - Jun 2024
Externally published	Yes

Keywords

Escherichia coli
proteome
SILAC
stationary phase

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10.1128/mra.00042-24

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title = "SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase",

abstract = "The expression level of individual proteins varies markedly during the progression of the growth phase in bacteria. A set of proteins was quantified in Escherichia coli total proteome during 14 days of batch cultivation using pulse stable isotope labeled amino acids in cell culture (SILAC)-based quantitative mass spectrometry.",

keywords = "Escherichia coli, proteome, SILAC, stationary phase",

author = "Kaspar Reier and Aivar Liiv and Jaanus Remme",

note = "Publisher Copyright: Copyright {\textcopyright} 2024 Reier et al.",

year = "2024",

month = jun,

doi = "10.1128/mra.00042-24",

language = "English",

volume = "13",

journal = "Microbiology Resource Announcements",

issn = "2169-8287",

publisher = "American Society for Microbiology",

number = "6",

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T1 - SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase

AU - Reier, Kaspar

AU - Liiv, Aivar

AU - Remme, Jaanus

PY - 2024/6

Y1 - 2024/6

N2 - The expression level of individual proteins varies markedly during the progression of the growth phase in bacteria. A set of proteins was quantified in Escherichia coli total proteome during 14 days of batch cultivation using pulse stable isotope labeled amino acids in cell culture (SILAC)-based quantitative mass spectrometry.

AB - The expression level of individual proteins varies markedly during the progression of the growth phase in bacteria. A set of proteins was quantified in Escherichia coli total proteome during 14 days of batch cultivation using pulse stable isotope labeled amino acids in cell culture (SILAC)-based quantitative mass spectrometry.

KW - Escherichia coli

KW - proteome

KW - SILAC

KW - stationary phase

UR - http://www.scopus.com/inward/record.url?scp=85197400300&partnerID=8YFLogxK

U2 - 10.1128/mra.00042-24

DO - 10.1128/mra.00042-24

M3 - Article

AN - SCOPUS:85197400300

SN - 2169-8287

VL - 13

JO - Microbiology Resource Announcements

JF - Microbiology Resource Announcements

IS - 6

ER -

SILAC analysis of Escherichia coli proteome during progression of growth from exponential to prolonged stationary phase

Abstract

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