TY - JOUR
T1 - Shedding light on the venom proteomes of the allergy-relevant hymenoptera Polistes dominula (European paper wasp) and Vespula spp. (Yellow Jacket)
AU - Grosch, Johannes
AU - Hilger, Christiane
AU - Bilò, Maria Beatrice
AU - Kler, Stephanie
AU - Schiener, Maximilian
AU - Dittmar, Gunnar
AU - Bernardin, François
AU - Lesur, Antoine
AU - Ollert, Markus
AU - Schmidt-Weber, Carsten B.
AU - Blank, Simon
N1 - Funding Information:
Conflicts of Interest: C.H. reports grants from Laboratoires Réunis, Luxembourg, and non-financial support from Macro Array Diagnostics, Vienna, outside this study. M.B.B. has received a speaker’s honorarium and consultancy fees from ALK-Abelló, outside the submitted work. M.O. reports personal fees from Thermo Fisher Scientific, Siemens Healthcare Diagnostics, Hitachi Chemical Diagnostics and Hycor, outside the submitted work; and is Scientific co-founder of the university biotech spin-off PLS-Design GmbH, Hamburg, Germany. C.B.S.-W. reports grants and personal fees from Bencard Allergie GmbH, grants from Leti Pharma, grants and personal fees from Allergopharma, grants and personal fees from PLS Design, outside the submitted work. S.B. reports non-financial support from ALK-Abelló, grants, personal fees and nonfinancial support from Bencard Allergie GmbH, personal fees from Teomed AG, grants from Leti Pharma, grants and personal fees from Thermo Fisher Scientific, grants from Allergy Therapeutics, outside the submitted work. The other authors declare that they have no conflict of interest.
Funding Information:
Funding: This study was supported by the Helmholtz Association, Future Topic “Immunology and Inflammation” (ZT-0027) to C.B.S.-W. and S.B.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/5
Y1 - 2020/5
N2 - Allergic reactions to stings of Hymenoptera species can have serious or even fatal consequences. If the identification of the culprit insect is possible, venom-specific immunotherapy effectively cures Hymenoptera venom allergies. Although component-resolved diagnostics has strongly evolved in recent years, the differentiation between allergies to closely related species such as Polistes dominula and Vespula spp. is still challenging. In order to generate the basis for new diagnostic and therapeutic strategies, this study aims at resolving the venom proteomes (venomes) of these species. The venoms of P. dominula and Vespula spp. (V. germanica, V. vulgaris) were analyzed by liquid chromatography-mass spectrometry. Resulting proteins were characterized regarding their function, localization and biochemical properties. The analyses yielded 157 proteins in Vespula spp. and 100 in P. dominula venom; 48 proteins, including annotated allergens, were found in both samples. In addition to a variety of venom trace molecules, new allergen candidates such as icarapin-like protein and phospholipase A2 were identified. This study elucidates the venomes of closely related allergy-eliciting Hymenoptera species. The data indicates that relying on marker allergens to differentiate between P. dominula and Vespula spp. venom allergy is probably insufficient and that strategies using cross-reactive major allergens could be more promising.
AB - Allergic reactions to stings of Hymenoptera species can have serious or even fatal consequences. If the identification of the culprit insect is possible, venom-specific immunotherapy effectively cures Hymenoptera venom allergies. Although component-resolved diagnostics has strongly evolved in recent years, the differentiation between allergies to closely related species such as Polistes dominula and Vespula spp. is still challenging. In order to generate the basis for new diagnostic and therapeutic strategies, this study aims at resolving the venom proteomes (venomes) of these species. The venoms of P. dominula and Vespula spp. (V. germanica, V. vulgaris) were analyzed by liquid chromatography-mass spectrometry. Resulting proteins were characterized regarding their function, localization and biochemical properties. The analyses yielded 157 proteins in Vespula spp. and 100 in P. dominula venom; 48 proteins, including annotated allergens, were found in both samples. In addition to a variety of venom trace molecules, new allergen candidates such as icarapin-like protein and phospholipase A2 were identified. This study elucidates the venomes of closely related allergy-eliciting Hymenoptera species. The data indicates that relying on marker allergens to differentiate between P. dominula and Vespula spp. venom allergy is probably insufficient and that strategies using cross-reactive major allergens could be more promising.
KW - Allergen
KW - European paper wasp
KW - Hymenoptera venom allergy
KW - Polistes dominula venom
KW - Venom proteome
KW - Vespula spp. venom
KW - Yellow jacket
UR - http://www.scopus.com/inward/record.url?scp=85084880872&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/32422898
U2 - 10.3390/toxins12050323
DO - 10.3390/toxins12050323
M3 - Article
C2 - 32422898
AN - SCOPUS:85084880872
SN - 2072-6651
VL - 12
JO - Toxins
JF - Toxins
IS - 5
M1 - 323
ER -