Selective isolation of bacterial DNA from human clinical specimens

Hans Peter Horz; Sebastian Scheer; Frank Huenger; Morgana E. Vianna; Georg Conrads

doi:10.1016/j.mimet.2007.10.007

Selective isolation of bacterial DNA from human clinical specimens

Hans Peter Horz^*, Sebastian Scheer, Frank Huenger, Morgana E. Vianna, Georg Conrads

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

67 Citations (Scopus)

Abstract

We evaluated two DNA preparation strategies (MolYsis®, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove®, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.

Original language	English
Pages (from-to)	98-102
Number of pages	5
Journal	Journal of Microbiological Methods
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2007.10.007
Publication status	Published - Jan 2008
Externally published	Yes

Keywords

Detection of human pathogens
Nucleic acid-based microbial diagnostic systems
Separation of human and bacterial DNA

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10.1016/j.mimet.2007.10.007

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title = "Selective isolation of bacterial DNA from human clinical specimens",

abstract = "We evaluated two DNA preparation strategies (MolYsis{\textregistered}, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove{\textregistered}, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.",

keywords = "Detection of human pathogens, Nucleic acid-based microbial diagnostic systems, Separation of human and bacterial DNA",

author = "Horz, {Hans Peter} and Sebastian Scheer and Frank Huenger and Vianna, {Morgana E.} and Georg Conrads",

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AU - Scheer, Sebastian

AU - Huenger, Frank

AU - Vianna, Morgana E.

AU - Conrads, Georg

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AB - We evaluated two DNA preparation strategies (MolYsis®, Molzym GmbH & Co. KG, Bremen, Germany) and Pureprove®, SIRS-Lab GmbH, Jena, Germany) to selectively extract bacterial DNA from human clinical samples. By testing 16 oral samples we found that human DNA could be largely eliminated while detectable levels of bacterial DNA were obtained with all samples. Both approaches hold great potential for microbial diagnostic systems.

KW - Detection of human pathogens

KW - Nucleic acid-based microbial diagnostic systems

KW - Separation of human and bacterial DNA

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Selective isolation of bacterial DNA from human clinical specimens

Abstract

Keywords

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