Selected reaction monitoring applied to quantitative proteomics.

Reiko Kiyonami*, Bruno Domon

*Corresponding author for this work

    Research output: Contribution to journalArticleResearchpeer-review

    14 Citations (Scopus)


    Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.

    Original languageEnglish
    Pages (from-to)155-166
    Number of pages12
    JournalMethods in Molecular Biology
    Publication statusPublished - 2010


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