Selected reaction monitoring applied to quantitative proteomics.

Reiko Kiyonami; Bruno Domon

doi:10.1007/978-1-60761-780-8_9

Selected reaction monitoring applied to quantitative proteomics.

Reiko Kiyonami^*, Bruno Domon

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

14 Citations (Scopus)

Abstract

Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.

Original language	English
Pages (from-to)	155-166
Number of pages	12
Journal	Methods in Molecular Biology
Volume	658
DOIs	https://doi.org/10.1007/978-1-60761-780-8_9
Publication status	Published - 2010

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title = "Selected reaction monitoring applied to quantitative proteomics.",

abstract = "Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.",

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AB - Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.

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Selected reaction monitoring applied to quantitative proteomics.

Abstract

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