Sample preparation and profiling: mass-spectrometry-based profiling strategies

Y. J. Kim, B. Domon

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


    In proteomics-based biomarker discovery, mass spectrometry (MS) has become an essential technology for both qualitative and quantitative analyses of proteins. In conjunction with separation methods, such as liquid chromatography (LC) or electrophoresis, the systematic peptide identification and quantification has expedited the profiling of protein components in biological samples. Numerous studies aiming at the discovery of clinically meaningful biomarker candidates have employed such MS-based proteome profiling approaches. The robustness of mass spectrometric methods has in addition facilitated large-scale analyses required for the verification and validation stages of biomarker discovery studies. In this chapter, LC-MS based profiling strategies for the discovery of protein biomarkers are discussed. In addition to reviewing the experimental strategies widely employed in the field, a specific example of biomarker discovery is discussed in this chapter.
    Original languageEnglish
    Title of host publicationComprehensive Biomarker Discovery and Validation for Clinical Application
    EditorsPéter Horvatovich, Rainer Bischoff
    Place of PublicationCambridge
    PublisherRSC Publishing
    Number of pages26
    VolumeRSC Drug Discovery series, 33
    ISBN (Print)978-1-84973-422-6
    Publication statusPublished - 10 Jun 2013


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