Saliva is a reliable and practical source of germline DNA for genome-wide studies in chronic lymphocytic leukemia

Silvia Rasi, Alessio Bruscaggin, Andrea Rinaldi, Stefania Cresta, Marco Fangazio, Lorenzo de Paoli, Sara Monti, Ernesto Gargiulo, Ivo Kwee, Robin Foà, Francesco Bertoni, Gianluca Gaidano, Davide Rossi*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

High-throughput genomics requires tumor DNA matched to germline DNA, that cannot be easily obtained in the context of leukemia. Using chronic lymphocytic leukemia as a model, saliva DNA was frequently devoid of tumor DNA also during overt disease, and passed quality controls for SNP-array (77/102, 75.4%) and next generation sequencing (71/102, 69.6%). Compared to saliva, urine provides germline DNA of similar quality but in lower amounts. Saliva DNA was successfully run on SNP 6.0 arrays, and passed quality control call rate thresholds. On these bases, saliva represents a useful source of germline DNA for high-throughput genomic studies of hematologic neoplasia.

Original languageEnglish
Pages (from-to)1419-1422
Number of pages4
JournalLeukemia Research
Volume35
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • Chronic lymphocytic leukemia
  • Germline DNA
  • Next generation sequencing
  • SNP array
  • Saliva

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