RT-PCR-assisted quantification of type I IFN responses in irradiated cancer cells

Claudia Galassi, Yangjingyi Ruan, Ai Sato, Carlos Jiménez-Cortegana, Vanessa Klapp, Norma Bloy, Emma Guilbaud, Giulia Petroni, Aitziber Buqué, Lorenzo Galluzzi*, Takahiro Yamazaki

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


It is now clear that radiation therapy (RT) can be delivered in doses and according to fractionation schedules that actively elicit immunostimulatory effects. While such effects are often sufficient to drive potent anticancer immunity culminating with systemic disease eradication, the immunostimulatory activity of RT stands out as a promising combinatorial partner for bona fide immunotherapeutics including immune checkpoint inhibitors (ICIs). Accumulating preclinical and clinical evidence indicates that the secretion of type I interferon (IFN) by irradiated cancer cells is a sine qua non for RT to initiate ICI-actionable tumor-targeting immune responses. Here, we detail a simple protocol to quantitatively assess type I IFN responses in irradiated mouse hormone receptor (HR)+ TS/A cells by RT-PCR. With minimal variations, the same technique can be straightforwardly adapted to quantify type I IFN-associated transcriptional responses in a variety of human and mouse cancer cells maintained in vitro.

Original languageEnglish
Title of host publicationRadiation Oncology and Radiotherapy Part A
EditorsAi Sato, Jeffrey Kraynak, Ariel E. Marciscano, Lorenzo Galluzzi
PublisherAcademic Press Inc.
Number of pages17
ISBN (Print)9780323899499
Publication statusPublished - Jan 2022
Externally publishedYes

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X


  • Breast cancer
  • CGAS
  • CXCL10
  • MX1
  • Mitochondrial DNA
  • PD-1
  • STING1
  • TS/A
  • Tumor immunology
  • Viral mimicry


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