Role of the nuclear transport factor p10 in nuclear import

Ulf Nehrbass, Günter Blobel*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

149 Citations (Scopus)

Abstract

The nuclear import factor p10 was cloned from Saccharomyces cerevisiae and found to be essential. The protein p10 can bind directly to several peptide repeat-containing nucleoporins. It also binds to the guanosine triphosphatase (GTPase) Ran in its guanosine diphosphate (GDP)-bound form and to karyopherin β. Assembly of the karyopherin heterodimer on immobilized nucleoporin yielded cooperative binding of p10 and Ran-GDP. Addition of GTP to this pentameric complex led to dissociation of karyopherin α, presumably via in situ formation of Ran-GTP from Ran-GDP. Thus, p10 appears to coordinate the Ran-dependent association and dissociation reactions underlying nuclear import.

Original languageEnglish
Pages (from-to)120-122
Number of pages3
JournalScience
Volume272
Issue number5258
DOIs
Publication statusPublished - 5 Apr 1996
Externally publishedYes

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