Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations

Stela S. Lazarova; Gaynor Malloch; Claudio M.G. Oliveira; Judith Hübschen; Roy Neilson

Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations

Stela S. Lazarova^*, Gaynor Malloch, Claudio M.G. Oliveira, Judith Hübschen, Roy Neilson

^*Corresponding author for this work

Clinical and Applied Virology

Research output: Contribution to journal › Article › Research › peer-review

105 Citations (Scopus)

Abstract

The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanum-group populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.

Original language	English
Pages (from-to)	404-410
Number of pages	7
Journal	Journal of Nematology
Volume	38
Issue number	4
Publication status	Published - Dec 2006

Keywords

18S rDNA
Longidorid
Longidoridae
Molecular analysis
Nematode
Taxonomy
mtDNA

Cite this

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title = "Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations",

abstract = "The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanum-group populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.",

keywords = "18S rDNA, Longidorid, Longidoridae, Molecular analysis, Nematode, Taxonomy, mtDNA",

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T1 - Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations

AU - Lazarova, Stela S.

AU - Malloch, Gaynor

AU - Oliveira, Claudio M.G.

AU - Hübschen, Judith

AU - Neilson, Roy

PY - 2006/12

Y1 - 2006/12

N2 - The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanum-group populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.

AB - The 18S ribosomal DNA (rDNA) and cytochrome oxidase I region of mitochondrial DNA (mtDNA) were sequenced for 24 Xiphinema americanum-group populations sourced from a number of geographically disparate locations. Sequences were subjected to phylogenetic analysis and compared. 18S rDNA strongly suggested that only X. pachtaicum, X. simile (two populations) and a X. americanum s.l. population from Portugal were different from the other 20 populations studied, whereas mtDNA indicated some heterogeneity between populations. Phylogenetically, based on mtDNA, an apparent dichotomy existed amongst X. americanum-group populations from North America and those from Asia, South America and Oceania. Analyses of 18S rDNA and mtDNA sequences underpin the classical taxonomic issues of the X. americanum-group and cast doubt on the degree of speciation within the X. americanum-group.

KW - 18S rDNA

KW - Longidorid

KW - Longidoridae

KW - Molecular analysis

KW - Nematode

KW - Taxonomy

KW - mtDNA

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M3 - Article

AN - SCOPUS:34147142846

SN - 0022-300X

VL - 38

SP - 404

EP - 410

JO - Journal of Nematology

JF - Journal of Nematology

IS - 4

ER -

Ribosomal and mitochondrial DNA analyses of Xiphinema americanum-group populations

Abstract

Keywords

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