Repetitive DNA sequences located in the central region of the human mdr1 (multidrug resistance) gene may account for a gene fusion event during its evolution

Marc Pauly; Isabelle Kayser; Martine Schmitz; Fernand Ries; François Hentges; Mario Dicato

doi:10.1007/BF00173178

Repetitive DNA sequences located in the central region of the human mdr1 (multidrug resistance) gene may account for a gene fusion event during its evolution

Marc Pauly^*, Isabelle Kayser, Martine Schmitz, Fernand Ries, François Hentges, Mario Dicato

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

The mdr1 gene, first member of the human multidrug-resistance gene family, is a major gene involved in cellular resistance to several drugs used in anticancer chemotherapy. Its product, the drug-excreting P-glycoprotein, shows a bipartite structure formed by two similar adjacent halves. According to one hypothesis, the fusion of two related ancestral genes during evolution could have resulted in this structure. The DNA sequence analysis of the introns located in the region connecting the two halves of the human mdr1 gene revealed a highly conserved poly(CA) · poly (TG) sequence in intron 15 and repeated sequences of the Alu family in introns 14 and 17. These repeated sequences most likely represent “molecular fossils” of ancient DNA elements which were involved in such a recombination event.

Original language	English
Pages (from-to)	974-978
Number of pages	5
Journal	Journal of Molecular Evolution
Volume	41
Issue number	6
DOIs	https://doi.org/10.1007/BF00173178
Publication status	Published - Dec 1995
Externally published	Yes

Keywords

Alu repeat
Conservation
DNA sequence
Gene evolution
Gene fusion
Human mdr1 gene
Intron
Multidrug resistance
P-glycoprotein
poly(CA) · poly(TG) microsatellite

Access to Document

10.1007/BF00173178

Cite this

@article{334742b0f1664824b649e61895f6b31e,

title = "Repetitive DNA sequences located in the central region of the human mdr1 (multidrug resistance) gene may account for a gene fusion event during its evolution",

abstract = "The mdr1 gene, first member of the human multidrug-resistance gene family, is a major gene involved in cellular resistance to several drugs used in anticancer chemotherapy. Its product, the drug-excreting P-glycoprotein, shows a bipartite structure formed by two similar adjacent halves. According to one hypothesis, the fusion of two related ancestral genes during evolution could have resulted in this structure. The DNA sequence analysis of the introns located in the region connecting the two halves of the human mdr1 gene revealed a highly conserved poly(CA) · poly (TG) sequence in intron 15 and repeated sequences of the Alu family in introns 14 and 17. These repeated sequences most likely represent “molecular fossils” of ancient DNA elements which were involved in such a recombination event.",

keywords = "Alu repeat, Conservation, DNA sequence, Gene evolution, Gene fusion, Human mdr1 gene, Intron, Multidrug resistance, P-glycoprotein, poly(CA) · poly(TG) microsatellite",

author = "Marc Pauly and Isabelle Kayser and Martine Schmitz and Fernand Ries and Fran{\c c}ois Hentges and Mario Dicato",

year = "1995",

month = dec,

doi = "10.1007/BF00173178",

language = "English",

volume = "41",

pages = "974--978",

journal = "Journal of Molecular Evolution",

issn = "0022-2844",

publisher = "Springer New York",

number = "6",

}

TY - JOUR

T1 - Repetitive DNA sequences located in the central region of the human mdr1 (multidrug resistance) gene may account for a gene fusion event during its evolution

AU - Pauly, Marc

AU - Kayser, Isabelle

AU - Schmitz, Martine

AU - Ries, Fernand

AU - Hentges, François

AU - Dicato, Mario

PY - 1995/12

Y1 - 1995/12

N2 - The mdr1 gene, first member of the human multidrug-resistance gene family, is a major gene involved in cellular resistance to several drugs used in anticancer chemotherapy. Its product, the drug-excreting P-glycoprotein, shows a bipartite structure formed by two similar adjacent halves. According to one hypothesis, the fusion of two related ancestral genes during evolution could have resulted in this structure. The DNA sequence analysis of the introns located in the region connecting the two halves of the human mdr1 gene revealed a highly conserved poly(CA) · poly (TG) sequence in intron 15 and repeated sequences of the Alu family in introns 14 and 17. These repeated sequences most likely represent “molecular fossils” of ancient DNA elements which were involved in such a recombination event.

AB - The mdr1 gene, first member of the human multidrug-resistance gene family, is a major gene involved in cellular resistance to several drugs used in anticancer chemotherapy. Its product, the drug-excreting P-glycoprotein, shows a bipartite structure formed by two similar adjacent halves. According to one hypothesis, the fusion of two related ancestral genes during evolution could have resulted in this structure. The DNA sequence analysis of the introns located in the region connecting the two halves of the human mdr1 gene revealed a highly conserved poly(CA) · poly (TG) sequence in intron 15 and repeated sequences of the Alu family in introns 14 and 17. These repeated sequences most likely represent “molecular fossils” of ancient DNA elements which were involved in such a recombination event.

KW - Alu repeat

KW - Conservation

KW - DNA sequence

KW - Gene evolution

KW - Gene fusion

KW - Human mdr1 gene

KW - Intron

KW - Multidrug resistance

KW - P-glycoprotein

KW - poly(CA) · poly(TG) microsatellite

UR - http://www.scopus.com/inward/record.url?scp=0029591607&partnerID=8YFLogxK

U2 - 10.1007/BF00173178

DO - 10.1007/BF00173178

M3 - Article

C2 - 8587143

AN - SCOPUS:0029591607

SN - 0022-2844

VL - 41

SP - 974

EP - 978

JO - Journal of Molecular Evolution

JF - Journal of Molecular Evolution

IS - 6

ER -

Repetitive DNA sequences located in the central region of the human mdr1 (multidrug resistance) gene may account for a gene fusion event during its evolution

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this