Redox modulation of the apoptogenic activity of thapsigargin

Claudia Cerella; Simona Coppola; Maria D'Alessio; Milena De Nicola; Andrea Magrini; Antonio Bergamaschi; Lina Ghibelli

doi:10.1196/annals.1387.028

Redox modulation of the apoptogenic activity of thapsigargin

Claudia Cerella, Simona Coppola, Maria D'Alessio, Milena De Nicola, Andrea Magrini, Antonio Bergamaschi, Lina Ghibelli^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Citations (Scopus)

Abstract

Thapsigargin (THG), a selective inhibitor of endoplasmic reticulum (ER) Ca²⁺-ATPases, causes the rapid emptying of ER Ca²⁺; in some cell types, this is accompanied by apoptosis, whereas other cells maintain viability. In order to understand the molecular determinants of such a different behavior, we explored the role of oxygen versus nitrogen radicals, by analyzing the apoptogenic ability of THG in the presence of inhibitors of glutathione or nitric oxide (NO) synthesis, respectively. We observed that oxygen radicals play a sensitizing role whereas nitrogen radicals prevent THG-dependent apoptosis, showing that the apoptogenic effect of THG is redox sensitive.

Original language	English
Title of host publication	Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion
Subtitle of host publication	Fifth International Conference
Publisher	Blackwell Publishing Inc.
Pages	469-472
Number of pages	4
ISBN (Print)	1573316490, 9781573316491
DOIs	https://doi.org/10.1196/annals.1387.028
Publication status	Published - Mar 2007
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1099
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Apoptosis
Calcium
Glutathione
Nitric oxide
Thapsigargin

Access to Document

10.1196/annals.1387.028

Cite this

Cerella, C., Coppola, S., D'Alessio, M., De Nicola, M., Magrini, A., Bergamaschi, A., & Ghibelli, L. (2007). Redox modulation of the apoptogenic activity of thapsigargin. In Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference (pp. 469-472). (Annals of the New York Academy of Sciences; Vol. 1099). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1387.028

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title = "Redox modulation of the apoptogenic activity of thapsigargin",

abstract = "Thapsigargin (THG), a selective inhibitor of endoplasmic reticulum (ER) Ca2+-ATPases, causes the rapid emptying of ER Ca2+; in some cell types, this is accompanied by apoptosis, whereas other cells maintain viability. In order to understand the molecular determinants of such a different behavior, we explored the role of oxygen versus nitrogen radicals, by analyzing the apoptogenic ability of THG in the presence of inhibitors of glutathione or nitric oxide (NO) synthesis, respectively. We observed that oxygen radicals play a sensitizing role whereas nitrogen radicals prevent THG-dependent apoptosis, showing that the apoptogenic effect of THG is redox sensitive.",

keywords = "Apoptosis, Calcium, Glutathione, Nitric oxide, Thapsigargin",

author = "Claudia Cerella and Simona Coppola and Maria D'Alessio and {De Nicola}, Milena and Andrea Magrini and Antonio Bergamaschi and Lina Ghibelli",

year = "2007",

month = mar,

doi = "10.1196/annals.1387.028",

language = "English",

isbn = "1573316490",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing Inc.",

pages = "469--472",

booktitle = "Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion",

}

Cerella, C, Coppola, S, D'Alessio, M, De Nicola, M, Magrini, A, Bergamaschi, A & Ghibelli, L 2007, Redox modulation of the apoptogenic activity of thapsigargin. in Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Annals of the New York Academy of Sciences, vol. 1099, Blackwell Publishing Inc., pp. 469-472. https://doi.org/10.1196/annals.1387.028

Redox modulation of the apoptogenic activity of thapsigargin. / Cerella, Claudia; Coppola, Simona; D'Alessio, Maria et al.
Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Blackwell Publishing Inc., 2007. p. 469-472 (Annals of the New York Academy of Sciences; Vol. 1099).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Redox modulation of the apoptogenic activity of thapsigargin

AU - Cerella, Claudia

AU - Coppola, Simona

AU - D'Alessio, Maria

AU - De Nicola, Milena

AU - Magrini, Andrea

AU - Bergamaschi, Antonio

AU - Ghibelli, Lina

PY - 2007/3

Y1 - 2007/3

N2 - Thapsigargin (THG), a selective inhibitor of endoplasmic reticulum (ER) Ca2+-ATPases, causes the rapid emptying of ER Ca2+; in some cell types, this is accompanied by apoptosis, whereas other cells maintain viability. In order to understand the molecular determinants of such a different behavior, we explored the role of oxygen versus nitrogen radicals, by analyzing the apoptogenic ability of THG in the presence of inhibitors of glutathione or nitric oxide (NO) synthesis, respectively. We observed that oxygen radicals play a sensitizing role whereas nitrogen radicals prevent THG-dependent apoptosis, showing that the apoptogenic effect of THG is redox sensitive.

AB - Thapsigargin (THG), a selective inhibitor of endoplasmic reticulum (ER) Ca2+-ATPases, causes the rapid emptying of ER Ca2+; in some cell types, this is accompanied by apoptosis, whereas other cells maintain viability. In order to understand the molecular determinants of such a different behavior, we explored the role of oxygen versus nitrogen radicals, by analyzing the apoptogenic ability of THG in the presence of inhibitors of glutathione or nitric oxide (NO) synthesis, respectively. We observed that oxygen radicals play a sensitizing role whereas nitrogen radicals prevent THG-dependent apoptosis, showing that the apoptogenic effect of THG is redox sensitive.

KW - Apoptosis

KW - Calcium

KW - Glutathione

KW - Nitric oxide

KW - Thapsigargin

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DO - 10.1196/annals.1387.028

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AN - SCOPUS:34248529733

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SN - 9781573316491

T3 - Annals of the New York Academy of Sciences

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BT - Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion

PB - Blackwell Publishing Inc.

ER -

Cerella C, Coppola S, D'Alessio M, De Nicola M, Magrini A, Bergamaschi A et al. Redox modulation of the apoptogenic activity of thapsigargin. In Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Blackwell Publishing Inc. 2007. p. 469-472. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1387.028

Redox modulation of the apoptogenic activity of thapsigargin

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