Quantitative proteomics using the high resolution accurate mass capabilities of the quadrupole-orbitrap mass spectrometer

Sebastien Gallien, Bruno Domon*

*Corresponding author for this work

    Research output: Contribution to journalArticleResearchpeer-review

    12 Citations (Scopus)

    Abstract

    High resolution/accurate mass hybrid mass spectrometers have considerably advanced shotgun proteomics and the recent introduction of fast sequencing capabilities has expanded its use for targeted approaches. More specifically, the quadrupole-orbitrap instrument has a unique configuration and its new features enable a wide range of experiments. An overview of the analytical capabilities of this instrument is presented, with a focus on its application to quantitative analyses. The high resolution, the trapping capability and the versatility of the instrument have allowed quantitative proteomic workflows to be redefined and new data acquisition schemes to be developed. The initial proteomic applications have shown an improvement of the analytical performance. However, as quantification relies on ion trapping, instead of ion beam, further refinement of the technique can be expected.

    Original languageEnglish
    Pages (from-to)2159-2170
    Number of pages12
    JournalBioanalysis
    Volume6
    Issue number16
    DOIs
    Publication statusPublished - 1 Aug 2014

    Fingerprint

    Dive into the research topics of 'Quantitative proteomics using the high resolution accurate mass capabilities of the quadrupole-orbitrap mass spectrometer'. Together they form a unique fingerprint.

    Cite this