Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Alex Steimle*, Erica T. Grant, Mahesh S. Desai

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

Original languageEnglish
Article number100326
Pages (from-to)100326
JournalSTAR Protocols
Volume2
Issue number1
DOIs
Publication statusPublished - 19 Mar 2021

Keywords

  • Metabolism
  • Protein expression and purification

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