Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Alex Steimle; Erica T. Grant; Mahesh S. Desai

doi:10.1016/j.xpro.2021.100326

Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Alex Steimle^*, Erica T. Grant, Mahesh S. Desai

^*Corresponding author for this work

Nutrition, Microbiome and Immunity

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

Original language	English
Article number	100326
Pages (from-to)	100326
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100326
Publication status	Published - 19 Mar 2021

Keywords

Metabolism
Protein expression and purification

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10.1016/j.xpro.2021.100326Licence: CC BY-NC-ND

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title = "Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples",

abstract = "The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).",

keywords = "Metabolism, Protein expression and purification",

author = "Alex Steimle and Grant, {Erica T.} and Desai, {Mahesh S.}",

note = "Funding Information: Work in the authors{\textquoteright} laboratory was supported by the following grants to M.S.D.: Luxembourg National Research Fund (FNR) CORE grants ( C15/BM/10318186 and C18/BM/12585940 ); FNR AFR bilateral grant ( 15/11228353 ); and FNR INTER Mobility grant ( 16/11455695 ). E.T.G. was supported by the following FNR grant: PRIDE17/11823097 . Publisher Copyright: {\textcopyright} 2021 The Author(s)",

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doi = "10.1016/j.xpro.2021.100326",

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AU - Steimle, Alex

AU - Grant, Erica T.

AU - Desai, Mahesh S.

N1 - Funding Information: Work in the authors’ laboratory was supported by the following grants to M.S.D.: Luxembourg National Research Fund (FNR) CORE grants ( C15/BM/10318186 and C18/BM/12585940 ); FNR AFR bilateral grant ( 15/11228353 ); and FNR INTER Mobility grant ( 16/11455695 ). E.T.G. was supported by the following FNR grant: PRIDE17/11823097 . Publisher Copyright: © 2021 The Author(s)

PY - 2021/3/19

Y1 - 2021/3/19

N2 - The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

AB - The gut microbiome expresses a multitude of enzymes degrading polysaccharides in dietary plant fibers and in host-secreted mucus. The quantitative detection of these glycan-degrading enzymes in fecal samples is important to elucidate the functional activity of the microbiome in health and disease. We describe a protocol for detection of glycan-degrading enzyme activity in mouse and human fecal samples, namely sulfatase and four carbohydrate-active enzymes. Assessing their activity can inform treatment strategies for diseases linked to the gut microbiome. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).

KW - Metabolism

KW - Protein expression and purification

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UR - https://pubmed.ncbi.nlm.nih.gov/33665621

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JO - STAR Protocols

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ER -

Quantitative assay to detect bacterial glycan-degrading enzyme activities in mouse and human fecal samples

Abstract

Keywords

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