Quantification of proteins in urine samples using targeted mass spectrometry methods

Nina Khristenko, Bruno Domon

    Research output: Contribution to journalArticleResearchpeer-review

    11 Citations (Scopus)


    Numerous clinical proteomics studies are focused on the development of biomarkers to improve either diagnostics for early disease detection or the monitoring of the response to the treatment. Although, a wealth of biomarker candidates are available, their evaluation and validation in a true clinical setup remains challenging. In biomarkers evaluation studies, a panel of proteins of interest are systematically analyzed in a large cohort of samples. However, in spite of the latest progresses in mass spectrometry, the consistent detection of pertinent proteins in high complex biological samples is still a challenging task. Thus, targeted LC-MS/MS methods are better suited for the systematic analysis of biomarkers rather than shotgun approaches. This chapter describes the workflow used to perform targeted quantitative analyses of proteins in urinary samples. The peptides, as surrogates of the protein of interest, are commonly measured using a triple quadrupole mass spectrometers operated in selected reaction monitoring (SRM) mode. More recently, the advances in targeted LC-MS/MS analysis based on parallel reaction monitoring (PRM) performed on a quadrupole-orbitrap instrument have allowed to increase the specificity and selectivity of the measurements.

    Original languageEnglish
    Pages (from-to)207-220
    Number of pages14
    JournalMethods in Molecular Biology
    Publication statusPublished - 2015


    • Mass spectrometry
    • Quantification
    • Selected reaction monitoring
    • Stable isotope-labeled (SIL) peptides
    • Targeted proteomics
    • Urine


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