TY - JOUR
T1 - Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers
AU - Grusdat, Melanie
AU - Dostert, Catherine
AU - Brenner, Dirk
N1 - Not indexed in PubMed
Funding Information:
The authors would like to thank the National Cytometry Platform, the Luxembourg Institute of Health's Animal Welfare Structure and the National Institutes of Health (NIH) Tetramer Core Facility. The cell lines VL-4, MC-57 and the LCMV strains, that have been used to establish this protocol, were kindly provided by Philipp A. Lang (University of Düsseldorf, Germany). The authors are supported by the FNRS Televie program (7459719F).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2023/1/16
Y1 - 2023/1/16
N2 - Lymphocytic choriomeningitis virus (LCMV) is a frequently used animal model to study immune responses against acute and chronic viral infections. LCMV is a non-cytopathic virus, but destruction of infected cells by cytotoxic T lymphocytes (CTLs) can lead to severe damage of tissues. Virus-specific T cell responses have to be balanced. A low virus load leads to a strong T cell response and subsequently to viral control. In contrast, a high viral titer is associated with T cell exhaustion and chronic viral infections. During an intermediate LCMV viral load CD8+ T cells can cause immunopathology, which can have detrimental outcomes. The LCMV infection model offers the opportunity to study virus-specific CD4+ and CD8+ T cell responses during chronic and acute infections by quantifying LCMV-specific T cells by tetramer staining and measuring cytokine production and viral titers in different organs.
AB - Lymphocytic choriomeningitis virus (LCMV) is a frequently used animal model to study immune responses against acute and chronic viral infections. LCMV is a non-cytopathic virus, but destruction of infected cells by cytotoxic T lymphocytes (CTLs) can lead to severe damage of tissues. Virus-specific T cell responses have to be balanced. A low virus load leads to a strong T cell response and subsequently to viral control. In contrast, a high viral titer is associated with T cell exhaustion and chronic viral infections. During an intermediate LCMV viral load CD8+ T cells can cause immunopathology, which can have detrimental outcomes. The LCMV infection model offers the opportunity to study virus-specific CD4+ and CD8+ T cell responses during chronic and acute infections by quantifying LCMV-specific T cells by tetramer staining and measuring cytokine production and viral titers in different organs.
KW - acute viral infection
KW - chronic viral infection
KW - Lymphocytic choriomeningitis virus
KW - T cell
UR - http://www.scopus.com/inward/record.url?scp=85130341446&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/36653079
U2 - 10.1016/bs.mcb.2022.03.003
DO - 10.1016/bs.mcb.2022.03.003
M3 - Article
C2 - 36653079
AN - SCOPUS:85130341446
SN - 0091-679X
VL - 173
SP - 121
EP - 131
JO - Methods in Cell Biology
JF - Methods in Cell Biology
ER -