Abstract
High sensitivity isoelectric focusing generates supernumerary lactate dehydrogenase bands. Using a two-dimensional electrophoresis/isoelectric focusing technique, we showed that the supernumerary bands co-migrate with the five isoenzyme bands in conventional agarose gel electrophoresis. Pyruvate, but not other physiological ligands plays a role in generating supernumerary bands, probably by binding to the enzyme.
| Original language | English |
|---|---|
| Pages (from-to) | 281-287 |
| Number of pages | 7 |
| Journal | Clinical Chemistry and Enzymology Communications |
| Volume | 3 |
| Issue number | 5 |
| Publication status | Published - 1990 |
| Externally published | Yes |