Pyruvate-induced supernumerary lactate dehydrogenase bands in isoelectric focusing

L. Seik, C. Schmidt, C. P. Muller

Research output: Contribution to journalArticleResearchpeer-review


High sensitivity isoelectric focusing generates supernumerary lactate dehydrogenase bands. Using a two-dimensional electrophoresis/isoelectric focusing technique, we showed that the supernumerary bands co-migrate with the five isoenzyme bands in conventional agarose gel electrophoresis. Pyruvate, but not other physiological ligands plays a role in generating supernumerary bands, probably by binding to the enzyme.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalClinical Chemistry and Enzymology Communications
Issue number5
Publication statusPublished - 1990
Externally publishedYes


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