Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation

V. Regnault; C. Rivat; L. Vallar; C. Geschier; J. F. stoltz

doi:10.1016/0378-4347(92)80013-G

Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation

V. Regnault^*, C. Rivat, L. Vallar, C. Geschier, J. F. stoltz

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

The application of a purification procedure for the industrial preparation from human plasma of a therapeutic protein may be hindered by several safety concerns. The dye leaching from Remazol Yellow GGL-Sepharose used for the affinity chromatography of human plasma transthyretin was quantitatively studied by a sensitive competitive enzyme immunoassay. The possibility of including a heat treatment step for virus inactivation in the purification process while preserving the biochemical and functional characteristics of the protein is also reported.

Original language	English
Pages (from-to)	93-99
Number of pages	7
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	584
Issue number	1
DOIs	https://doi.org/10.1016/0378-4347(92)80013-G
Publication status	Published - 11 Dec 1992
Externally published	Yes

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10.1016/0378-4347(92)80013-G

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Regnault, V., Rivat, C., Vallar, L., Geschier, C., & stoltz, J. F. (1992). Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation. Journal of Chromatography B: Biomedical Sciences and Applications, 584(1), 93-99. https://doi.org/10.1016/0378-4347(92)80013-G

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title = "Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation",

abstract = "The application of a purification procedure for the industrial preparation from human plasma of a therapeutic protein may be hindered by several safety concerns. The dye leaching from Remazol Yellow GGL-Sepharose used for the affinity chromatography of human plasma transthyretin was quantitatively studied by a sensitive competitive enzyme immunoassay. The possibility of including a heat treatment step for virus inactivation in the purification process while preserving the biochemical and functional characteristics of the protein is also reported.",

author = "V. Regnault and C. Rivat and L. Vallar and C. Geschier and stoltz, {J. F.}",

note = "Funding Information: This work was partly supported by a grant from M.R.T. (France).",

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doi = "10.1016/0378-4347(92)80013-G",

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Regnault, V, Rivat, C, Vallar, L, Geschier, C & stoltz, JF 1992, 'Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 584, no. 1, pp. 93-99. https://doi.org/10.1016/0378-4347(92)80013-G

Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation. / Regnault, V.; Rivat, C.; Vallar, L. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 584, No. 1, 11.12.1992, p. 93-99.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Purification of biologically active human plasma transthyretin by dye-affinity chromatography

T2 - studies on dye leakage and possibility of heat treatment for virus inactivation

AU - Regnault, V.

AU - Rivat, C.

AU - Vallar, L.

AU - Geschier, C.

AU - stoltz, J. F.

N1 - Funding Information: This work was partly supported by a grant from M.R.T. (France).

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Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation

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