TY - JOUR
T1 - PTPN13 and β-catenin regulate the quiescence of hematopoietic stem cells and their interaction with the bone marrow niche
AU - López-Ruano, Guillermo
AU - Prieto-Bermejo, Rodrigo
AU - Ramos, Teresa L.
AU - San-Segundo, Laura
AU - Sánchez-Abarca, Luis Ignacio
AU - Sánchez-Guijo, Fermín
AU - Pérez-Simón, José Antonio
AU - Sánchez-Yagüe, Jesús
AU - Llanillo, Marcial
AU - Hernández-Hernández, Ángel
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/10/13
Y1 - 2015/10/13
N2 - The regulation of hematopoietic stem cells (HSCs) depends on the integration of the multiple signals received from the bone marrow niche. We show the relevance of the protein tyrosine phosphatase PTPN13 and β-catenin as intracellular signaling molecules to control HSCs adhesiveness, cell cycling, and quiescence. Lethally irradiated mice transplanted with Lin- bone marrow cells in which PTPN13 or β-catenin had been silenced showed a significant increase of long-term (LT) and short-term (ST) HSCs. A decrease in cycling cells was also found, together with an increase in quiescence. The decreased expression of PTPN13 or β-catenin was linked to the upregulation of several genes coding for integrins and several cadherins, explaining the higher cell adhesiveness. Our data are consistent with the notion that the levels of PTPN13 and β-catenin must be strictly regulated by extracellular signaling to regulate HSC attachment to the niche and the balance between proliferation and quiescence.
AB - The regulation of hematopoietic stem cells (HSCs) depends on the integration of the multiple signals received from the bone marrow niche. We show the relevance of the protein tyrosine phosphatase PTPN13 and β-catenin as intracellular signaling molecules to control HSCs adhesiveness, cell cycling, and quiescence. Lethally irradiated mice transplanted with Lin- bone marrow cells in which PTPN13 or β-catenin had been silenced showed a significant increase of long-term (LT) and short-term (ST) HSCs. A decrease in cycling cells was also found, together with an increase in quiescence. The decreased expression of PTPN13 or β-catenin was linked to the upregulation of several genes coding for integrins and several cadherins, explaining the higher cell adhesiveness. Our data are consistent with the notion that the levels of PTPN13 and β-catenin must be strictly regulated by extracellular signaling to regulate HSC attachment to the niche and the balance between proliferation and quiescence.
UR - http://www.scopus.com/inward/record.url?scp=84944441467&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2015.08.003
DO - 10.1016/j.stemcr.2015.08.003
M3 - Article
C2 - 26344907
AN - SCOPUS:84944441467
SN - 2213-6711
VL - 5
SP - 516
EP - 531
JO - Stem Cell Reports
JF - Stem Cell Reports
IS - 4
ER -