Protocol to screen for Sorafenib resistance regulators using pooled lentiviral shRNA library and a Sorafenib-resistant hepatocellular carcinoma cell model

Ruize Gao*, Fengyuan Tang, Gerhard Christofori

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Approaches to study therapy resistance in HCC (hepatocellular carcinoma) are limited, especially when using HCC models in vitro. Here, we present a protocol to establish an in vitro Sorafenib-resistant human HCC cell model and conduct an shRNA-mediated synthetic lethal screen in established Sorafenib-resistant HCC cell lines to identify critical regulators of Sorafenib resistance. We describe steps for RNA sequencing and functional analysis to reveal the mode of action of potential candidates in conferring therapy resistance to HCC cells. For complete details on the use and execution of this protocol, please refer to Gao et al. (2021a)1 and Gao et al. (2021b).2

Original languageEnglish
Article number102273
JournalSTAR Protocols
Volume4
Issue number2
Early online date30 Apr 2023
DOIs
Publication statusPublished - 16 Jun 2023
Externally publishedYes

Keywords

  • Cancer
  • Cell Biology
  • High Throughput Screening
  • Molecular Biology
  • RNAseq
  • Sequencing

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