@article{21674f78acd348239744d7e1616efbfa,
title = "Protocol for the generation and assessment of functional macrophages from mouse bone marrow cells",
abstract = "Macrophages play essential roles in tissues, wherein they exert beneficial or detrimental functions depending on the signals they encounter during their differentiation. Here, we present a protocol to differentiate mouse bone marrow cells into macrophages under specific cues to evaluate their impact on macrophage phenotypic acquisition. We provide detailed instructions for optimal cell culture conditions, quality controls, and examples of concluding immunological functional assays. This protocol is applicable in short- and long-term drug-based modulation of macrophage functions. For complete details on the use and execution of this protocol, please refer to Scafidi et al.1",
keywords = "Cell biology, Cell culture, Cell isolation, Immunology, Model organisms, Molecular biology",
author = "Andrea Scafidi and {Lind-Holm Mogensen}, Frida and Alessandro Michelucci",
note = "Acknowledgments We acknowledge financial support from the Action LIONS Vaincre le Cancer Luxembourg. F.L.-H.M. was supported by the Luxembourg National Research Fund (FNR) through the FNR-PRIDE program i2TRON (PRIDE/14254520/I2TRON). We would like to acknowledge Mr. Alexandros Pailas and Ms. Marta De Lucas Sanz for the introduction to CellProfiler, as well as Dr. Djalil Coowar at the University of Luxembourg Animal Facility and Ms. Ana{\"i}s Oudin and Ms. Stephanie Sallai at the Luxembourg Institute of Health Animal Facility for the technical support with the collection of mouse samples. Lastly, we thank Dr. Aur{\'e}lie Poli for assisting with the setting up of the functional analyses and Ms. Amandine Bernard for helping with the key resources table. For the purpose of open access, and in fulfilment of the obligations arising from the FNR grant agreement, the author has applied a Creative Commons Attribution 4.0 International (CC BY 4.0) license to any Author Accepted Manuscript version arising from this submission. The graphical abstract and the figures were created using BioRender.com Publisher Copyright: {\textcopyright} 2025 The Author(s)",
year = "2025",
month = mar,
day = "20",
doi = "10.1016/j.xpro.2025.103706",
language = "English",
volume = "6",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}