Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

Laura Morin; Brice Autier; Patrice Hemon; Simon Le Gallou; Mikael Roussel; Sarah Dion; Valérie Lecureur

doi:10.1016/j.xpro.2025.103684

Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

Laura Morin, Brice Autier, Patrice Hemon, Simon Le Gallou, Mikael Roussel, Sarah Dion, Valérie Lecureur^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Cytometry by time of flight (CyTOF) is a flow cytometry-based technique using metal-tagged antibodies, allowing immunophenotyping. Here, we present a protocol for phenotyping mouse myeloid and lymphoid cells isolated from lung, spleen, and intraperitoneal lavages in healthy or pathologic conditions. We describe steps for antibody labeling and titration, tissue dissociations, and staining. We then detail procedures to compare staining obtained from fresh or cryopreserved tissues. For complete details on the use and execution of this protocol, please refer to Morin et al.¹

Original language	English
Article number	103684
Journal	STAR Protocols
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2025.103684
Publication status	Published - 21 Mar 2025
Externally published	Yes

Keywords

cell isolation
mass cytometry
single cell

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10.1016/j.xpro.2025.103684Licence: CC BY

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abstract = "Cytometry by time of flight (CyTOF) is a flow cytometry-based technique using metal-tagged antibodies, allowing immunophenotyping. Here, we present a protocol for phenotyping mouse myeloid and lymphoid cells isolated from lung, spleen, and intraperitoneal lavages in healthy or pathologic conditions. We describe steps for antibody labeling and titration, tissue dissociations, and staining. We then detail procedures to compare staining obtained from fresh or cryopreserved tissues. For complete details on the use and execution of this protocol, please refer to Morin et al.1",

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AU - Morin, Laura

AU - Autier, Brice

AU - Hemon, Patrice

AU - Le Gallou, Simon

AU - Roussel, Mikael

AU - Dion, Sarah

AU - Lecureur, Valérie

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Cytometry by time of flight (CyTOF) is a flow cytometry-based technique using metal-tagged antibodies, allowing immunophenotyping. Here, we present a protocol for phenotyping mouse myeloid and lymphoid cells isolated from lung, spleen, and intraperitoneal lavages in healthy or pathologic conditions. We describe steps for antibody labeling and titration, tissue dissociations, and staining. We then detail procedures to compare staining obtained from fresh or cryopreserved tissues. For complete details on the use and execution of this protocol, please refer to Morin et al.1

AB - Cytometry by time of flight (CyTOF) is a flow cytometry-based technique using metal-tagged antibodies, allowing immunophenotyping. Here, we present a protocol for phenotyping mouse myeloid and lymphoid cells isolated from lung, spleen, and intraperitoneal lavages in healthy or pathologic conditions. We describe steps for antibody labeling and titration, tissue dissociations, and staining. We then detail procedures to compare staining obtained from fresh or cryopreserved tissues. For complete details on the use and execution of this protocol, please refer to Morin et al.1

KW - cell isolation

KW - mass cytometry

KW - single cell

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UR - https://pubmed.ncbi.nlm.nih.gov/40067826/

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JO - STAR Protocols

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ER -

Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry

Abstract

Keywords

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