@article{d3e9a7f3eac04a2ea172f066a5d26954,
title = "Protocol for isolating leukemia-derived extracellular vesicles from the spleen of preclinical models of leukemia using ultracentrifugation",
abstract = "Here, we present a protocol for the direct isolation of small extracellular vesicles (sEVs) from the spleen of preclinical murine models of leukemia using ultracentrifugation. We describe steps for tissue collection, sample preparation, ultracentrifugation-based isolation, and sEV characterization. This protocol allows for efficient enrichment of both leukemia and its microenvironment-derived sEV (LME-sEV), providing a valuable tool for studying their composition and functional roles. Potential applications include investigating the role of sEV in leukemia progression and identifying biomarkers. For complete details on the use and execution of this protocol, please refer to Gargiulo et al.1",
keywords = "cancer, cell biology, cell separation/fractionation",
author = "Ernesto Gargiulo and Morande, {Pablo Elias} and Maxmilan Jeyakumar and Lucie Rospape and J{\'e}r{\^o}me Paggetti and Etienne Moussay",
note = "Acknowledgments We thank Pr. Carlo Croce and Pr. John Byrd (Ohio State University) for the kind gift of Eμ-TCL1 mouse, provided by Dr. Martina Seiffert (DKFZ Heidelberg, Germany). This work was supported by grants from the Luxembourg National Research Fund (FNR) to E.G., L.R., M.J., E.M., and J.P. (PRIDE15/10675146/CANBIO, PRIDE21/16763386/CANBIO2, INTER/DFG/16/11509946, C20/BM/14582635, and C20/BM/14592342) and from the European Commission to P.E.M. (H2020-MSCA-IF-2020: 101029602). Copyright {\textcopyright} 2024 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2024",
month = sep,
day = "20",
doi = "10.1016/j.xpro.2024.103244",
language = "English",
volume = "5",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",
}