@article{15e4154b31174088a78f613173cd0c5f,
title = "Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors",
abstract = "Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing. For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).",
keywords = "Cancer, Cell Biology, Cell culture, Cell isolation, Model Organisms, Neuroscience, Organoids",
author = "Ana{\"i}s Oudin and Virginie Baus and Vanessa Barthelemy and Carina Fabian and Eliane Klein and Monika Dieterle and May Wantz and Hau, {Ann Christin} and Claire Dording and Amandine Bernard and Alessandro Michelucci and Yabo, {Yahaya A.} and Georgia Kanli and Olivier Keunen and Rolf Bjerkvig and Niclou, {Simone P.} and Anna Golebiewska",
note = "Funding Information: We are grateful to the Neurosurgery Department of the Centre Hospitalier de Luxembourg, Laboratoire National de Sant{\'e}, and Clinical and Epidemiological Investigation Center (CIEC) of the LIH for support in tumor collection. We acknowledge the financial support by the Luxembourg Institute of Health , T{\'e}l{\'e}vie-FNRS (grants GBModImm n°7.8513.18 and TETHER n°7.4615.18 ), the Luxembourg National Research Fund (FNR; CANBIO DTU PRIDE15/10675146 ), and the GLIOTRAIN ITN funded by the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement no. 766069 . Funding Information: We are grateful to the Neurosurgery Department of the Centre Hospitalier de Luxembourg, Laboratoire National de Sant?, and Clinical and Epidemiological Investigation Center (CIEC) of the LIH for support in tumor collection. We acknowledge the financial support by the Luxembourg Institute of Health, T?l?vie-FNRS (grants GBModImm n?7.8513.18 and TETHER n?7.4615.18), the Luxembourg National Research Fund (FNR; CANBIO DTU PRIDE15/10675146), and the GLIOTRAIN ITN funded by the European Union's Horizon 2020 research and innovation programme under the Marie Sk?odowska-Curie grant agreement no. 766069. V. Barthelemy, E.K. M.D. M.W. C.F. C.D. A.B. R.B. S.P.N. and A.G. optimized patient tumor processing and organoid biobanking. A.O. V. Baus, and S.P.N. optimized in vivo experiments. E.K. A.-C.H, A.B. A.M. Y.A.Y. and A.G. optimized tissue processing and organoid derivation for functional assays. A.O. G.K. and O.K. optimized MRI protocol. R.B. and S.P.N. provided initial protocols. A.M. O.K. R.B. S.P.N. and A.G. supervised the work and obtained funding. A.O. and A.G. prepared the first manuscript draft. All authors reviewed and edited the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100534",
language = "English",
volume = "2",
pages = "100534",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}